StemPro 34 SFM是什么培养基？

yangpan521

如题 以前看文献碰到StemPro
34 SFM培养基和STO培养体系，但具体是指什么？还没搞明白，哪位可以给予详细答案。谢谢

Drturtle

回复 yangpan521 的帖子$ {' b5 X+ q8 g+ u

. H9 G( u& ~) W8 t4 JStemPro 34 SFM是一中专门为血液干细胞而设的无血清培养基。6 e6 d3 W7 `9 E7 F1 ~
http://products.invitrogen.com/ivgn/product/10639011
5 E& b9 v: \9 }% ?7 _! r' X
* X, L: ]$ }, U& ]9 `4 x6 ~/ g  Y2 C3 R: R# q
至于STO feeders， 请看相关文献的描述：
  `' P% K0 h2 D& U, G; }# @4 H8 sSeveral alternative cell lines have been investigated
8 A3 l' E) B+ X; o9 hfor their ability to support existing hESC lines as well9 @; \" S* l% F, F  s/ f7 M
as being used to derive new hESC lines (Table 1). The' N8 q  _2 Z+ E! _* y, E2 K
mouse embryonic fibroblast cell line,STO, has been used9 O1 ]' ^1 ~5 O3 B7 ]
to establish nine cell lines from frozen blastocysts and& |  T; s* h+ C/ b7 h. A5 I
zygotes (Park et al., 2004). The advantage of STO cells/ E" H- A& o4 ?" ^  \
over primary cultures of MEFs is that, being immortalized,( J  w0 [) H  o4 c" Y8 x& v; H
they are easy to maintain and propagate. Human
! X: E2 e$ J2 ~) Y" aES cells cultured on this feeder layer exhibited a similar' L* R0 Z, T4 B6 _! ]
doubling time to those on MEFs and expressed surface
9 O$ [8 H) @) m; a; |: ^3 @6 W" Smarkers as expected. In addition, prolonged culture did9 B1 D/ M+ ~; }& e8 h
not lead to abnormal karyotypes. However, Xu et al.4 [! W5 L4 \. @! e" K
(2001) have found increased differentiation when conditioned
4 c) ?: ?& y- n: y# M- c5 emedium (CM) from these cells was used in
# N& {( E! q. C0 M0 wfeeder-free conditions. Thus, the STO line is not a direct
, X. @4 F$ ~% ^8 D& y' _0 ^3 Dsubstitute for MEFs but can reduce some of the work
* [$ P% V  X2 l- [  Wload regarding MEF isolation and culture.

yangpan521

非常感谢  受教了