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- 积分
- 50
- 威望
- 50
- 包包
- 191
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本书主要内容： s" R/ Q& \; v( c8 {
1. Making Protein Immunoprecipitates
O/ X3 e4 ^+ p2 c( h2 _6 B6 u2. Signal Transduction Inhibitors in Cellular Function
" q1 E0 a0 X9 y4 D- d1 z3. Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets" Q9 u* W$ Y( a
4. A High-Throughput Mammalian Cell-Based Transient Transfection Assay
2 S. y$ K8 Y. r. u S% Y) M- [7 a5. Determining Protein Half-Lives
0 R) w' C/ v% [ Z6. Assaying Protein Kinase Activity
& i6 J/ G! B* S' F, r' i7. Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach$ U6 U+ A9 _, x% I9 A. z1 b1 l
8. Studies of Calmodulin-Dependent Regulation$ o+ X* X4 |# I5 L
9. Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation& A, Z# y( [" X7 p! |
10. Characterization of Protein-DNA Assocation In Vivo by Chromatin Immunoprecipitation! g4 \8 S9 I/ O# O: f
11. Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins3 }7 P$ S3 ~. v( l$ r
12. Nucleocytoplasmic Glycosylation, O-GlcNAc( } l" D c: B
13. Techniques in Protein Methylation
2 u5 \4 D ~' Z( I9 M# r( J14. Assaying Lipid Phosphate Phosphatase Activities: r: ]. W, V2 b3 L0 [2 F
15. Assaying Phosphoinositide Phosphatases. S- K" d# j6 F
16. Assaying Phospholipase A2 Activity/ X' K2 {5 M* @! f
17. Measurement and Immunofluorescence of Cellular Phosphoinositides9 @! v" }* L9 N( u' e
18. Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer+ F5 f d7 S. u. k( d5 M4 v
19. In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET! ]9 T/ K6 X( q( E4 h# D& P
20. Revealing Protein Dynamics by Photobleaching Techniques
/ P; b0 H: d/ ?3 I21. Assaying Cytochrome c Translocation During Apoptosis |
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总评分: 威望 + 6
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发表于 2010-7-14 12:12
