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- 积分
- 50
- 威望
- 50
- 包包
- 191
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本书主要内容：- R" P) \) e3 N, Z2 Z
1. Making Protein Immunoprecipitates
6 ^# b x i: t$ L* @4 J1 N% i2. Signal Transduction Inhibitors in Cellular Function
# \1 O: o1 n3 G+ G# ^/ v) t3. Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets
4 {. Y# K' w1 _& B4. A High-Throughput Mammalian Cell-Based Transient Transfection Assay
D: C) E; E9 c. `+ T/ t5. Determining Protein Half-Lives# P p" E4 @1 ]: h
6. Assaying Protein Kinase Activity
# g0 Q4 d1 ^. }6 {2 G" X# s7. Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach
0 @8 y+ d7 `# |8. Studies of Calmodulin-Dependent Regulation
+ [, ^; W2 N9 Z( z% ^; y9. Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation* E2 P' F, y. f7 }+ d8 d
10. Characterization of Protein-DNA Assocation In Vivo by Chromatin Immunoprecipitation
) n. l+ j) y" {7 m+ Y: B( z11. Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
. @* w$ _! G% q12. Nucleocytoplasmic Glycosylation, O-GlcNAc
- H9 ?% K( A; H W6 Q$ O" V$ [13. Techniques in Protein Methylation
+ J5 w$ y% {4 S# ?; ~$ s+ h: d; a14. Assaying Lipid Phosphate Phosphatase Activities
% [+ \6 F! c' z15. Assaying Phosphoinositide Phosphatases
0 g" t, V& C7 N+ }; Z16. Assaying Phospholipase A2 Activity' u+ s& {7 ?, v
17. Measurement and Immunofluorescence of Cellular Phosphoinositides% N( A, M7 r, y' Q U' A( s
18. Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer: k- c5 M0 ?% A( D* R. `7 A
19. In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
" _" E0 m) H' o20. Revealing Protein Dynamics by Photobleaching Techniques6 L' r% a# t) T$ `
21. Assaying Cytochrome c Translocation During Apoptosis |
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发表于 2010-7-14 12:12
