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本帖最后由 细胞海洋 于 2015-9-17 12:45 编辑 3 k+ Y5 o/ G8 U
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Contents, E1 a2 i# t4 a0 v9 J
Preface............................................................. v, b- o: S/ Z) f" U+ v
Contributors......................................................... xi- @0 T4 d: u2 [
1 Cancer Biology ................................................... 17 P% m: b ?1 Y3 Q, g8 `# q( b
Ian A. Cree
1 m' `% _$ O* U) r8 }- X 2 Principles of Cancer Cell Culture ..................................... 13
' g& f- ~9 G7 d) b* ^9 KIan A. Cree
# s b5 Y& S0 N w: i 3 Storage of Cell Lines............................................... 27+ K7 Z1 t* J# V- a- H0 Q) A
Katharine A. Parker
# b9 o7 p5 u4 t2 V 4 Characterization and Authentication of Cancer Cell Lines: An Overview ........ 355 R( x! o, ?6 i3 k6 `
Yvonne A. Reid * Z/ }4 o9 S8 ?4 a
5 Online Verification of Human Cell Line Identity by STR DNA Typing ......... 456 \. Y Z$ M& p. F5 y1 @
Wilhelm G. Dirks and Hans G. Drexler 7 o: t: a; s+ w8 d0 R/ V% J
6 Cytogenetic Analysis of Cancer Cell Lines............................... 57
/ }! C5 ?7 i0 _4 NRoderick A.F. MacLeod, Maren Kaufmann, and Hans G. Drexler
. ]) C5 d- }5 e ^ 7 Cell Culture Contamination ......................................... 79
7 q, ~2 G. Z. q% V9 f2 r7 iGlyn N. Stacey 2 P v$ b5 X# l$ U
8 Detecting Mycoplasma Contamination in Cell Cultures
4 D4 T% t+ U" M$ \by Polymerase Chain Reaction ....................................... 93
7 K2 U- t1 V( r- H8 zCord C. Uphoff and Hans G. Drexler
, N$ P0 @/ P3 T; l$ x 9 Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics ........ 1054 m0 s- G8 X! Q1 E' J/ y" o7 H4 P
Cord C. Uphoff and Hans G. Drexler
1 R' A+ c6 [/ ^ Z5 t a$ c y10 Quality Assurance and Good Laboratory Practice ......................... 115
. u! \8 y8 X4 s9 g1 W* E0 hLouise A. Knight and Ian A. Cree ; l- T- h3 _# o. o" F- f% ~
11 Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning .... 125( p% u* Z, J9 e2 a9 v* R
Laura Breen, Joanne Keenan, and Martin Clynes / Q& i% K4 f: I4 @! X! u; O
12 Isolation and Culture of Colon Cancer Cells and Cell Lines ................. 1350 T! w3 v5 {8 r; j' d$ l$ r
Sharon Glaysher and Ian A. Cree
0 \: N! k. b# h; j6 y. y13 Isolation and Culture of Melanoma and Naevus Cells and Cell Lines........... 1411 Y6 A5 @) b" y' V
Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett 0 |& R& ~- s: l# ]5 Y8 y* M( ^
14 Isolation and Culture of Squamous Cell Carcinoma Lines ................... 151
, Y7 q; f4 Y( |1 t- hKarin J. Purdie, Celine Pourreyron, and Andrew P. South " Q8 R$ h4 Z$ t% d8 u9 `2 Y
15 Isolation and Culture of Ovarian Cancer Cells and Cell Lines ................ 161, J* m; A! V& w6 W4 V2 Q* E
Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, 5 U5 v: M! a$ t }
Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz
2 H9 F1 Y* c1 J, o0 K' N$ I# D16 Establishment and Culture of Leukemia–Lymphoma Cell Lines .............. 181
% r1 i# q( n, _Hans G. Drexler * W I; R" b1 h& T7 M
17 Isolation of Inflammatory Cells from Human Tumours..................... 2016 J3 ^ X) S9 K9 l t, p
Marta E. Polak 4 n3 s6 }0 C! ~5 S% V
18 Isolation of Endothelial Cells from Human Tumors ....................... 209
J+ c% q* e+ E* P4 Q1 ~Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau,
# b2 D/ n# A, b3 u/ `1 @Roland S. Croner, and Michael Stürzl 8 Q# b* Y' S9 D& w F r8 O& r
19 Cellular Chemosensitivity Assays: An Overview ........................... 219
" U4 D7 F5 R- G6 K" t1 wVenil N. Sumantran
+ W! y) M2 P+ b4 _& G20 Cell Sensitivity Assays: The MTT Assay ................................. 237
" ?: s: P6 A8 @$ ^7 X1 \0 a' VJohan van Meerloo, Gertjan J.L. Kaspers, and Jacqueline Cloos
, G% n ?0 Y! X. [7 z/ w1 q21 Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay ......... 247
: o3 ~' ?5 ]- D" \% J7 d, oSharon Glaysher and Ian A. Cree
: H+ `: b& I- J; A6 m22 Differential Staining Cytotoxicity Assay: A Review ........................ 2592 t6 {9 M" q+ k. B( }; n' W- t
Larry M. Weisenthal . P6 s9 r$ E& D* ]+ ^
23 Real-Time Cytotoxicity Assays ....................................... 2851 k. M! j, d, y3 A
Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz,
# i2 D" X5 B0 k0 }/ g- mand Jonathan M. Cooper , p7 O" N6 @" y6 u6 Z
24 Purification of Annexin V and Its Use in the Detection of Apoptotic Cells ...... 293
: F! q6 @" y+ N9 x$ V$ Z* t$ Q# QKaty M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss
Z5 R% R% C+ Q3 j2 [25 Measurement of DNA Damage in Individual Cells Using the Single Cell Gel
+ A) T* j0 p5 I6 k EElectrophoresis (Comet) Assay ....................................... 3095 R+ E% }; P" z' I9 m& ~
Janet M. Hartley, Victoria J. Spanswick, and John A. Hartley 3 k1 ?) w+ T/ i+ d# N. _
26 Molecular Breakpoint Analysis of Chromosome Translocations 0 u- _3 G& G5 |* h
in Cancer Cell Lines by Long Distance Inverse-PCR ....................... 321/ k! l8 M( o% R$ U1 j4 {
Björn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod
$ D D& r- b" Q1 o0 \& x27 Cell Migration and Invasion Assays .................................... 333
' n9 M D9 N1 }; Z4 iKarwan A. Moutasim, Maria L. Nystrom, and Gareth J. Thomas ) K1 g" [+ O5 N: N
28 Angiogenesis Assays ............................................... 345' H# s2 N l3 H+ e- p. p
V. Poulaki
) O- Y% J+ y6 g29 Flow Cytometric DNA Analysis of Human Cancers and Cell Lines ............ 359
# p- v( E% R3 l7 p; PSarah A. Krueger and George D. Wilson - F8 Q2 T) N+ u3 p
30 Expression Analysis of Homeobox Genes in Leukemia/
u% v' l( p" x# o; lLymphoma Cell Lines ............................................. 371
9 y0 g+ |9 a0 K1 S6 f: l4 Y' ~Stefan Nagel and Hans G. Drexler 1 E: V' i) q0 c% A% Z' U
31 Measuring Gene Expression from Cell Cultures by Quantitative
$ N% `! B4 }5 Z. j3 x0 P4 f3 ~) mReverse-Transcriptase Polymerase Chain Reaction......................... 381
6 G: g7 J& }3 ]+ h2 f2 jSharon Glaysher, Francis G. Gabriel, and Ian A. Cree ! s' O: ~! n% U; [+ D1 W+ B
32 Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins .... 3950 `4 d9 d# y7 W# J9 l
Samantha Larkin and Claire Aukim-Hastie ' e3 |# A$ o! l0 t
33 Development of Rituximab-Resistant B-NHL Clones: An In Vitro ! R, K& R# T$ j
Model for Studying Tumor Resistance to Monoclonal Antibody-Mediated : w$ Q" a2 y+ J' t5 d1 L1 o
Immunotherapy .................................................. 407
# T) P9 A0 H1 E$ b) Z8 Y& IAli R. Jazirehi and Benjamin Bonavida
7 D2 E! O7 l2 \) i- i34 Analysis of Drug Interactions ........................................ 421; I8 b1 G i( N+ Y
Irene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters
- n% V: u& z' X- M6 B35 Transfection and DNA-Mediated Gene Transfer .......................... 435. y, ^3 Z8 ]. \/ W8 ?1 Z" @
Davide Zecchin and Federica Di Nicolantonio ! X6 E$ _) z3 M
ixContents, a8 m6 s# s( z8 ~2 `4 D3 R6 ^
36 Drug Design and Testing: Profiling of Antiproliferative
2 Q9 V. j3 V1 _6 b6 ]Agents for Cancer Therapy Using a Cell-Based Methyl-[3H]-
# I- D$ M& z! j# P! b" m- M' eThymidine Incorporation Assay ...................................... 451
7 O/ r% J& X/ \4 f8 s$ L6 C9 kMatthew Griffiths and Hardy Sundaram
; H0 D4 @1 c8 O7 U3 s37 Feeder Layers: Co-culture with Nonneoplastic Cells ....................... 467& B$ }! O, m7 I( M" y1 ?+ X3 |
Celine Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South \# _' {3 P) n. V' r" ^4 `0 j" V
38 Xenotransplantation of Breast Cancers ................................. 471
/ P! c7 p1 Z1 B5 cMassimiliano Cariati, Rebecca Marlow, and Gabriela Dontu 7 R* c( I7 E5 H y! S% M4 d0 g, P
Appendix A ......................................................... 483
( \% U- ]9 q& Q; l1 ?1 QAppendix B ......................................................... 487 R! j- h9 t* P1 a! H
Index .............................................................. 493 |
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发表于 2015-8-7 19:42
