关于间充质干细胞分化的问题

liluli0715

请各位高手帮忙下，如果MSC在培养过程中已经分化成脂肪干细胞，有没有相应的抗体可以检出两者区别？目前已检出出CD14，CD45，CD79a，HLA-DR，CD34，CD90，CD73，CD105不能区分MSC和脂肪干细胞。

yaolinzhang

回复 liluli0715 的帖子* f) t7 A6 h0 O" i' ^# Q' p
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The multipotent stem cells within adipose tissue, termed adipose-derived stem cells (ASCs). Phenotypically, the characterization of ASCs is still in its infancy. To date, all attempts to establish an exact definition of the ASC phenotype and to discriminate clearly between these cells and fibroblasts have been unsuccessful." \0 w& Z9 W+ c
As indicated, ASCs share many cell surface markers with pericytes and bone marrow-MSCs. Except for those mentioned above, the pericyte markers expressed by ASCs include
* k4 N; e, y! msmooth muscleb-actin, platelet-derived growth factor (PDGF) receptor-b, and neuro-glial proteoglycan 2 [25], while the markers shared by ASCs and MSCs include CD13, CD29,2 k* s, h! n2 z/ ]3 T
CD44, CD58, and CD166.
2 C8 N6 \5 j5 u, @/ \$ n; XHere is the paper may help you.. Y6 c; s" h3 q) g/ v3 C7 O, z
Concise Review: Adipose-Derived Stem Cells as a Novel Tool for Future Regenerative Medicine

liluli0715

thanks