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Mayor/Elsevier( k9 t6 q6 ^3 d0 a: K8 c0 v
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Tiny but dynamic domains define the elusive lipid raft, based on results from Madan Rao, Satyajit Mayor (National Centre for Biological Science, Bangalore, India), and colleagues.) M! z" G$ Q- O/ M! D
/ f1 L: A1 |! R) L7 i8 `/ ZDescribing the structure and components of rafts〞membrane domains enriched in specific lipids and proteins〞has been a long-standing challenge for cell biologists. In this new report, the authors use FRET, photobleaching, and theoretical modeling to get the closest look yet at raft components called GPI-APs (GPI-anchored proteins). They show that lipid rafts contain small clusters (four or fewer molecules) of very tight-knit GPI-APs packed into an 4-nm-wide space.
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About a third of any given GPI-AP species is found in rafts. The rest remain as monomers. This percentage holds true across multiple expression levels, which is inconsistent with equilibrium-based formation. "It means rafts have to be actively maintained," says Mayor. "And within these regions, the GPI proteins form clusters."8 N$ ]5 e/ y4 y* [' z9 K- N: a
# c% j" Q& D" [+ B0 b4 V$ W0 dDifferent types of GPI-APs are found within a cluster, and the clusters are dynamic〞cross-linking of one species removes it from the cluster, and another species readily takes its place. The cross-linked GPI-APs formed larger groups that were endocytosed by the clathrin-mediated pathway, rather than the route responsible for uptake of raft GPI-APs. Thus, ligation of a receptor could change its fate by altering its lipid environment.0 _2 k# e7 g: z) }/ J4 _( Z2 B
! V9 \0 l4 A- L3 W6 x$ oReference:
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Sharma, P., et al. 2004. Cell. 116:577–589.(Native clusters (black circles) reorgani) |
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发表于 2009-3-6 08:24
发表于 2015-6-18 19:26
