《自然》[Nature]13年5月16日出版pdf全文

nero1981

$ j* D% B& Y6 y$ R+ ?A three-dimensional view of intact mouse hippocampus rendered transparent, showing neurons expressing eYFP (green), parvalbumin (red) and glial fibrillary acidic protein (blue). High-resolution imaging of biological tissue has traditionally required sectioning, which for tissues like the brain means the loss of long-range connectivity. Now Karl Deisseroth and colleagues have developed a way of making full, intact organs optically transparent and macromolecule-permeable by building a hydrogel-based infrastructure from within the tissue that allows subsequent removal of light-scattering lipids, resulting in a transparent brain. The method, termed CLARITY, also allows repeated antibody labelling of proteins, and in situ hybridization of nucleic acids in non-sectioned tissue, such as full mouse brains or human clinical samples stored in formalin for many years. Cover: Kwanghun Chung & Karl Deisseroth, HHMI/Stanford Univ.
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