PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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) K: t$ B: _( H/ N% O5 DSpringer MMB Vol931 2013 共29章 552pages6 m* G6 c1 K2 O" x$ c
Cell Imaging Techniques  Methods and Protocols' j. @5 A( N% W: W8 T: @
Second Edition+ e' ^1 E8 N3 H( J- m6 O7 u' ]  }
Edited by
7 W% x9 E7 Q- v/ U" NDouglas J. Taatjes' C6 V) D" t% e

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Contents
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii
# a6 u, M. N0 q0 T, cContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii
1 ]! R7 c: T3 q. f) t$ t+ G1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       1
9 G2 U2 y' Q+ W6 k$ \" |2 N4 ~" iDouglas W. Cromey
) y' k( x" A) X- ~2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29
, S" h& j* }) y- CDonna J. Webb and Claire M. Brown7 J& i* b' _: c3 X3 Q" r
3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   613 K& g8 ]$ [3 U- c# |2 X
J. Lacoste, K. Young, and Claire M. Brown
+ O- O$ G( l8 T# w7 |, q0 M4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic $ U, s& ^2 |$ q
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 858 e) H( P/ s  D* V) u6 K
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill8 P9 {4 j6 O- D- F. o& ?& \
5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97
6 X9 S: N$ U! f1 |+ \/ oJeremy Adler and Ingela Parmryd5 V( l  A4 f! }8 ~8 q
6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    1112 L; g. v, ?+ Z; @; I* ?8 ]
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman; b3 }4 p+ c$ S# ^0 g
7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
' \1 L- K, g9 {# p# G+ Z1 ]! O4 S( TRaju Tomer, Khaled Khairy, and Philipp J. Keller4 w9 @+ G* k: y7 T5 Y
8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1397 H6 f$ z4 ~& j. f+ @2 V3 \( P
Lasse Evensen, Wolfgang Link, and James B. Lorens# e( E& ]" k/ m& Z1 C: [
9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153- F/ F) }1 R! C7 O+ P- p' V# |. [
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert9 K3 ]: N. p' s4 h* ?- m: ^
10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential , s- ^% K: J# B! w1 g- \! m
Interference$ H8 E4 q1 Y9 {3 n& r7 }% X& Q
Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169
2 f8 D/ @# V. H+ n9 H* T3 qWei Sun, Lehui Xiao, and Ning Fang
; L2 U  X/ s- [% B' a# V11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187
* {% \, X+ \, {( E0 B+ m& n1 ?" ~Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
' w4 D  t# K% \& W5 }12   Laser Capture Microdissection for Protein
4 k+ y3 K4 C7 j! i) g9 C$ B6 {and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2133 h! _: q% Z- e! l* `8 O# B* T
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,
' h* D3 [, l! [6 a9 r" jLance A. Liotta, and Virginia Espina
5 @: q! [- V( N13   Viewing Dynamic Interactions of Proteins and a Model Lipid
" K5 \, G# z. w0 j; t% gMembrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259% N6 U7 e$ H8 h( `
Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes- ]$ G# K+ H( \
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
) _* {. w) w9 @, b' @' {, TLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko5 `5 g* K+ {& U; l* `
15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
2 E- D) @3 ]% B' S" m6 E0 H# @+ |( dJoanna L. MacKay and Sanjay Kumar
7 o9 V, x  k  K. q/ T1 J16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
) B/ M) N' k/ WLilia A. Chtcheglova and Peter Hinterdorfer
# J" d0 |+ p' ]17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345- q$ o" z5 b+ h* Q7 B+ w2 q0 B5 R0 ]7 f
Bhanu P. Jena
- a7 Z2 F3 \% I" C9 h; I/ A18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367
+ h( t- {9 x& g. g3 K- UChristian Mühlfeld, Lars Knudsen, and Matthias Ochs8 ?( v" l* n' u% b6 J% ?
19 A Novel Combined Imaging/Morphometrical Method for the Analysis
1 j' Y# `0 n; `1 Z! {of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    391
( y& U( l# [) a. l1 s% qMichele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes/ R: B2 A9 b" c4 @( M7 Q/ h
20   Correlative Light–Electron Microscopy as a Tool to Study) I6 q4 Z4 `/ x+ P& E- g6 M/ g: I
In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413
, {: g  e* G1 Y5 D  w: U& fElena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini( t* D; e! n; K3 e, N# |1 P
21   Photooxidation Technology for Correlative Light5 I& y' U0 j/ |0 }+ B
and Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423
1 p& M/ J3 D' Q5 X/ \/ q: mClaudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, - q7 P6 c8 N4 {  _
Margit Pavelka, and Adolf Ellinger% P/ f1 V( S. F5 O
22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437, K, \  K( z. s7 k$ H  U  \( `
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and : ]& k6 L. u7 `+ y4 c
Margit Pavelka
; v+ |# `; ]$ P23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     449
' b/ b, }$ G5 l8 |Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori
: y1 H! c, \( H1 x# x24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467/ l- K8 Y) h& z7 e' k! Z
Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader. B8 [8 O( Y3 c
25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     4835 g2 u/ n( y4 X$ ^/ ^
Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto; d0 v/ v- N6 i, s
26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    493
1 @9 `* d  y1 \9 M, l" y6 xJ.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald. l1 I, n% d3 G. j/ N/ Z
27   Environmental Scanning Electron Microscopy Gold
3 i0 o/ D3 p! X  \) L% |/ _% ]  pImmunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517
, c4 i+ N1 x% sFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
6 }+ d( o; M) q  T28   High-Pressure Freezing for Scanning Transmission Electron
* z% L; S2 r8 M& K% y+ }$ tTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     525
/ j; C( u! }- s# D* c- [; hPaul Walther, Eberhard Schmid, and Katharina Höhn" q3 P% {* v4 d, F" G& d- h, W
29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     537' z$ v4 C  O/ I( |% ~& V
Stephan Meding and Axel Walch
+ x6 e4 \, R0 S  c$ V4 N; Y* @Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547, Q/ X  l; `% k
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