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本帖最后由 flowguo 于 2012-10-11 10:53 编辑 & | _* k) z" V3 ?- u/ w
. p/ g- y. @+ ~' ^9 L, x1 Y% cDendritic cell protocols
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edited by Shalin H. Naik. — 2nd ed. ) s( C. v( q" }, N
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Contents2 n f0 j q4 `* b6 E' }% ]6 D
3 Q7 w! E7 }/ f! ` Foreword- x; s6 r/ i g0 m2 x( ^
Contributors& Q1 n2 L4 Z( K: t5 F+ [1 A
SECTION I REVIEWS% b* K$ L+ ~4 c" \, v: m3 O( z
1. Review of Human DC Subtypes
5 j' l% w: r- d6 j7 { 2. Review of Murine Dendritic Cells: Types, Location, and Development 21
C# Q( Q- c: s+ X SECTION II HUMAN DC PROTOCOLS 43
6 g) U5 _2 H1 A, M* d2 V 3. Isolation of Human Blood DC Subtypes
$ P8 p4 X: f4 C! \3 t 4. Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin 554 S+ s! ^2 G+ m- p( z$ ~0 U5 q4 S' P% {
5. Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells 673 S3 B2 k. H* W0 ]$ u, Y9 M
6. In Vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2-/-%-/- Mice& a6 M& `/ d- `- R
7. Generation of Stable Th1/CTL-, Th2-, and Thl7-Inducing Human Dendritic Cells& ?: _7 F8 f8 f$ x8 x$ L
8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation. J; | y* M# v' {
9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines 149% L$ b+ k" L5 A
SECTION III MURINE DENDRITIC CELL METHODS 165
( B; @5 k9 L5 K6 e* _* _+ m( f 10. CD8+, CD8-, and Plasmacytoid Dendritic Cell Generation In Vitro Using fit3 Ligand 167) {% y; o3 f/ B/ O! v1 Q' g
11. Generation of Large Numbers of Pro-DCs and Pre-DCs In Vitro 177
' p0 }0 b1 z6 U3 g9 c% @ 12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF 1870 M% O" r8 F* M; I: f& P2 Y
13. Isolation of Common Dendritic Cell Progenitors (CDP) from Mouse Bone Marrow 195
. O; u: s9 i+ M" `5 ^. j( Z1 F! z 14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry 2058 y! `8 {9 L0 ]$ o1 ?1 i3 }5 K* J
15. Isolation of Cutaneous Dendritic Cells 231
' P/ M! P7 d4 V& g- y J/ M 16. Isolation of Skin Dendritic Cells from Mouse and Man 235
+ s+ U1 A! J5 ]4 r7 t# S( p: u% m1 p 17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells 249
! Z( r) m7 @. [& m- _1 i3 F( z3 t# P* l 18. Isolation of Mouse Thymic Dendritic Cell Precursors 265
. l; p5 L( ]1 X; Q1 y 19. Isolation of Rat Intestinal Lymph DC 2818 u5 \7 r! d& G) ?
20. Mouse Models of Viral Infection: Influenza Infection in the Lung 299
# W: V5 ]" V; A; G& ?' \& g, s 21. DCs in Mouse Models of Intracellular Bacterial Infection 319
3 B+ v% }3 C, [/ _. @ 22. Studying the Function of Dendritic Cells in Mouse Models of Asthma 331) ]+ o" c5 u8 \( I3 v2 l9 m- v
23. Direct Ex Vivo Activation of T cells for Analysis of Dendritic Cells Antigen Presentation 3514 R4 C; h C" p0 V/ B9 ?
24. Methods to Study Pulmonary Dendritic Cell Migration. a) l& q3 q3 ^4 ]1 [# K
25. Measuring pH, ROS Production, Maturation, and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays 383
/ O7 @2 N# T" p# s/ K6 M5 i9 x( z 26. Adaptive Treg Generation by DCs and Their Functional Analysis 4037 ^$ q+ U$ g! _# }
27. Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation 413
+ z& T$ ]- \3 _4 n/ [' Q 28. Defining In Vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice# L1 g9 x, h% e# ?4 y
Subject Index 443
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