dominant negative

mansnoopy

请教一下 dominant negative 是什么意思 ？ 具体的解释

jessewill

负显性，相当于是artificial构造的一个蛋白，这个蛋白对于target protein是显性，这样就block掉了target protein的作用，相当于knockdown了

jessewill

负显性，相当于是artificial构造的一个蛋白，这个蛋白对于target protein是显性，这样就block掉了target protein的作用，相当于knockdown了

zzc2211

Dominant negative mutation
' ^) l' e! }- t, J' CDefinition(s)7 x& t# \: m7 Z4 P; o) @8 Y: D
A mutation whose gene product adversely affects the normal, wild-type gene product within the same cell, usually by dimerizing (combining) with it. In cases of polymeric molecules, such as collagen, dominant negative mutations are often more deleterious than mutations causing the production of no gene product (null mutations or null alleles). . v" A* d. O1 p" }* d8 R
Definition from: GeneTests from the University of Washington and the National Center for Biotechnology Information" w, h" l4 L9 h/ \( _5 j( l

& ]3 M$ Q& X3 W3 r$ n, n5 zDominant negative mutations (also called antimorphic mutations) have an altered gene product that acts antagonistically to the wild-type allele. These mutations usually result in an altered molecular function (often inactive) and are characterised by a dominant or semi-dominant phenotype.
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2 r& f& m- L' j/ V# B7 x4 f4 G3 ODefinition of dominant negative :/ l& h1 o% }5 k" q
A mutation whose gene product adversely affects the normal, wild-type gene product within the same cell. This usually occurs if the product can still interact with the same elements as the wild-type product, but block some aspect of its function.
0 M, Z5 y. H7 Q. {8 O( ]Examples: , U. G4 R$ C3 t4 ?3 r
1. A mutation in a transcription factor that removes the activation domain, but still contains the DNA binding domain. This product can then block the wild-type transcription factor from binding the DNA site leading to reduced levels of gene activation.
8 }3 O4 ^* i6 ~, L( ?, g8 o6 t2. A protein that is functional as a dimer. A mutation that removes the functional domain, but retains the dimerization domain would cause a dominate negative phenotype, because some fraction of protein dimers would be missing one of the functional domains.' `- X! I; ~! U+ ]0 Z  c" [5 j
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dominant negative effect6 W! o; t; D5 j3 n, _5 u
显性负效应：某些信号转导蛋白突变后不仅自身无功能，还能抑制或阻断同一细胞内的野生型信号转导蛋白的作用，这种作用被称为显性负效应。具有显性负效应的突变体被称为显性负性突变体（dominant negative mutant)。机理是突变型蛋白和相关蛋白形成无功能的二聚体，野生型蛋白功能被抑制。2 M" o/ g9 E! Z

sciencezhu

简单的说就是你转进去（通常是过表达）的蛋白（突变型）抑制了体内正常蛋白的功能！

zzc2211

Dominant negative mutation
8 w1 j# \  P  FDefinition(s)  Z/ P0 [: P" d2 r, Q  n/ L
A mutation whose gene product adversely affects the normal, wild-type gene product within the same cell, usually by dimerizing (combining) with it. In cases of polymeric molecules, such as collagen, dominant negative mutations are often more deleterious than mutations causing the production of no gene product (null mutations or null alleles). $ ^( e1 F; z( R4 D
Definition from: GeneTests from the University of Washington and the National Center for Biotechnology Information
) k2 Z% B. Q9 ?! S" @  N
$ u, r/ Q$ C5 P  n" VDominant negative mutations (also called antimorphic mutations) have an altered gene product that acts antagonistically to the wild-type allele. These mutations usually result in an altered molecular function (often inactive) and are characterised by a dominant or semi-dominant phenotype.
# Y, k2 i' M$ C6 F: K0 G9 Z; d9 N% |
: l5 k: @: N4 Y4 bDefinition of dominant negative :6 H" _, n+ T2 ~- H2 c/ j3 O
A mutation whose gene product adversely affects the normal, wild-type gene product within the same cell. This usually occurs if the product can still interact with the same elements as the wild-type product, but block some aspect of its function.
) @4 p6 A) i4 m3 ?Examples:
2 t2 X% G% C1 s, L9 }7 E# R1. A mutation in a transcription factor that removes the activation domain, but still contains the DNA binding domain. This product can then block the wild-type transcription factor from binding the DNA site leading to reduced levels of gene activation.; G3 u: q7 O2 c6 H' P& J
2. A protein that is functional as a dimer. A mutation that removes the functional domain, but retains the dimerization domain would cause a dominate negative phenotype, because some fraction of protein dimers would be missing one of the functional domains.  v! |, p. P2 c1 M( P
: V) z- O4 F/ u7 j# H. y
dominant negative effect" V) [( ^0 m- e7 Q: C& @( F
显性负效应：某些信号转导蛋白突变后不仅自身无功能，还能抑制或阻断同一细胞内的野生型信号转导蛋白的作用，这种作用被称为显性负效应。具有显性负效应的突变体被称为显性负性突变体（dominant negative mutant)。机理是突变型蛋白和相关蛋白形成无功能的二聚体，野生型蛋白功能被抑制。
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mansnoopy

回复 zzc2211 的帖子
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( p1 f9 H* \! u- M+ N0 {7 kthank you so much

胡君

简单一点就是 蛋白是这种蛋白，但是其没有功能。有结构有序列没功能