耐辐射细胞株的建立的方法

jitianxing7021

Stem Cells：首次证实放疗能将乳腺癌细胞转变为癌干细胞! n- Q% j# G7 Q( X, m
Radiation-induced Reprograming of Breast Cancer Cells
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Chann Lagadec, Erina Vlashi, Lorenza Della Donna, Carmen Dekmezian, Frank Pajonk+ ?, W1 w" N$ D* Y; t

, D2 p; R( x7 S" l! C  cBreast cancers are thought to be organized hierarchically with a small number of breast cancer stem cells (BCSCs) able to re-grow a tumor while their progeny lack this ability. Recently, several groups reported enrichment for BCSCs when breast cancers were subjected to classical anticancer treatment. However, the underlying mechanisms leading to this enrichment are incompletely understood.% v! E) w8 H. P/ U4 L. z+ j; D
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Using non-BCSCs sorted from patient samples, we found that ionizing radiation reprogrammed differentiated breast cancer cells into induced BCSCs (iBCSCs). iBCSCs showed increased mammosphere formation, increased tumorigenicity and expressed the same stemness-related genes as BCSCs from non-irradiated samples. Reprogramming occurred in a polyploid subpopulation of cells, coincided with re-expression of the transcription factors Oct4, Sox-2, Nanog, and Klf4, and could be partially prevented by Notch inhibition.' x$ |5 U  k+ t  F& A2 j) g
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We conclude that radiation may induce a BCSC phenotype in differentiated breast cancer cells and that this mechanism contributes to increased BCSC numbers seen after classical anti-cancer treatment.
2 u7 V7 M; k9 v. v7 b' Z( H请问大家有没有建立耐抗肿瘤药物或者辐射的细胞株的经验和资料？以供大家相处学习！

leisong12

http://www.ncbi.nlm.nih.gov/pubmed/20935265+ _& R7 N3 i6 E/ a
http://jnci.oxfordjournals.org/content/102/21/1637.full.pdf