PDF电子书：Protein expression in mamallion cells （Humana Press 2012）

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! r' r5 v2 Y- T METHODS   IN  MOLECULAR  BIOLOGY* P4 L( {# _2 P& t  p5 T2 Y
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Protein Expression in Mammalian Cells' A! M, R( U. C! T2 I" r; b
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Methods and Protocols" A& f5 H+ M, S5 X7 m+ f
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Edited by  v7 l1 ^- a' U
James L. Hartley
( N. S& H' X0 z! P: K4 _Protein Expression Laboratory, SAIC-Frederick, Inc., National Cancer Institute, $ \- S3 B: Q8 b0 X: N3 U
Frederick, MD, USA
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Springer Science+Business Media, LLC 2012. W, o& s& M  \( N- r

1 u! R# I6 k7 a. q9 n4 V, M9 zContents
2 k: |  ~4 a, x0 x8 \/ z5 JContents% B/ G. n, }4 _( |( Z
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  v
/ `7 H4 K* l* H  [& ~+ aContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  ix3 q& e( y# n+ |! C  L
  1  Why Proteins in Mammalian Cells? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1
; A9 v4 H' b+ g( pJames L. Hartley
9 _5 K: m( p* b/ m( h3 W' t& I  2  Large-Scale Transfection of Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  13/ c- I: X& X1 G+ S# {
Lucia Baldi, David L. Hacker, Carine Meerschman,
: ^# \6 W; Y4 j  @  Q7 I$ o/ z" n8 ~: rand Florian M. Wurm
3 @: A+ o7 W" z' k) j  3  Selection of High Expressing Mammalian Cells by Surface Display & I* o; D( Y7 [; x5 P9 x
of Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  27; W% k6 {1 v2 P
Christine T. DeMaria
9 k2 J$ `# E' u# J0 D  R; I  4  Expression of a Secreted Protein in Mammalian Cells Using . z' ]! ^, v! q* c
Baculovirus Particles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  41
' o( {6 p, k  @2 O$ CBarbara Ann Jardin, Cynthia B. Elias, and Satya Prakash' h2 S9 [6 I, B
  5  Transfection of Difficult-to-Transfect Primary Mammalian Cells . . . . . . . . . . . . . . .  65
" T+ V4 z: h$ o; \9 d5 aOliver Gresch and Ludger Altrogge
: F& P; W1 z8 n; N$ \2 q  6  Stable Protein Expression in Mammalian Cells Using Baculoviruses  . . . . . . . . . . . .  750 V. H) _& J, m6 V" q: D# c, r2 S
Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, 3 ~- z5 E+ S0 g: m* i# u  [
Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch
$ z* `4 e) p& ]' \; v9 x5 r! A  7  Using Matrix Attachment Regions to Improve Recombinant
- O% e1 T; n& y' ~  x0 zProtein Production . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  93
' p$ |6 ^3 f6 y8 `5 l7 cNiamh Harraghy, Montserrat Buceta, Alexandre Regamey,
# L0 J& D0 [5 `4 wPierre-Alain Girod, and Nicolas Mermod! C) h+ W3 j# ^- v: O  J" g
  8  Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells  . . . . .  111( w* i3 }$ B, q0 E# @
Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh,
+ n7 ]' ]5 ?4 ?: N% B8 Pand Tina M. Sauerwald' r9 R; x! q8 J! b
  9  Post-transcriptional Regulatory Elements for Enhancing Transient Gene 2 r9 l% U" q& _$ i
Expression Levels in Mammalian Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  125# X* u1 V; C1 U0 f
Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang5 b2 G' X( M  n" a4 N# I- n
10  Converting Monoclonal Antibodies into Fab Fragments for Transient
; c+ h- I5 L/ e' @, B/ t( [Expression in Mammalian Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
5 H; X' b( ^. F3 U% G# UJoanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq,
/ {$ ^, T7 r$ ^6 m0 H' oRebecca Hamer, and Raymond J. Owens
: D: V: M. D# S6 g/ h11  Generation of High-Expressing Cells by Methotrexate Amplification
; U& I( Z6 d3 @3 ]: j( c8 Nof Destabilized Dihydrofolate Reductase Selection Marker  . . . . . . . . . . . . . . . . . . .  161, e) j; m7 a% ]5 W. K* u7 d
Say Kong Ng: l2 z; Z4 g( b9 r4 L
12  Tools for Coproducing Multiple Proteins in Mammalian Cells  . . . . . . . . . . . . . . . .  173
' ~. r5 P4 D$ k, b. ^$ `& oZahra Assur, Wayne A. Hendrickson, and Filippo Mancia. ?* J4 }$ ~* L* c0 a$ V
viii Contents
# J- t+ t" \. F4 B1 M: o13  Identification and Characterization of Protein Glycosylation Using Specific . T) L7 H  F2 e
Endo- and Exoglycosidases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  189
" m; i3 r' h3 Y& ?5 nPaula Magnelli, Alicia Bielik, and Ellen Guthrie" V* N: g6 Q( W) Q# c. C# w9 W
14  Strategies for Efficient Transfection of CHO-Cells with Plasmid DNA  . . . . . . . . . .  213+ \% v( a/ q. P! ?! B
Renate Kunert and Karola Vorauer-Uhl1 s& l8 f8 I9 X
15  Methods for Constructing Clones for Protein Expression in Mammalian Cells  . . . .  227" U  s' r3 o$ b
Takefumi Sone and Fumio Imamoto
4 ^9 T+ n" O' J2 f2 e16  Optimizing Transient Recombinant Protein Expression in Mammalian Cells . . . . . .  251
" p3 @# j3 f) T* PRalph F. Hopkins, Vanessa E. Wall, and Dominic Esposito
( O5 z2 h9 k  \0 O# ]" ]) a- gIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  269
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mimimi2256

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Why Proteins in Mammalian Cells

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