- 积分
- 394
- 威望
- 394
- 包包
- 1792
|
Trypsin: 胰蛋白酶的作用是使细胞间的蛋白质水解从而使细胞离散。使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。终止消化时,可用含有血清培养液或者胰酶抑制剂终止胰酶对细胞的作用。
! ?% d5 p/ z) C5 o0 w5 e0 U/ M详细信息见连接:http://baike.baidu.com/view/354286.htm
- i! i% z& A! x! O) r
' K5 ]5 ?/ t6 I2 t; OAccutase:用于细胞的消化时,与Trypsin相比较:Accutase 再完成消化后,不需要抑制剂;节省时间。Accutase的温和的消化,能增加细胞存活率。" X( C, C1 D* m/ |6 `4 D
详细信息见:
Accutase.pdf
(649.07 KB)
$ R- O1 e$ \$ C N
7 O: L8 j0 n+ }- }) u9 S+ u9 j+ g: R" n4 ^; Y) t
Dispase is a protease that is suitable for the gentle dissociation of a wide variety of tissues. Incubation of minced tissue with pre-warmed dispase and gentle agitation will liberate cells with minimal cell damage. Pre-warmed dispase can also be used to harvest cells from tissue culture plastic. Unlike trypsin, dispase is not inhibited by serum. Dispase activity is inhibited by EDTA and EGTA. Dispase should be removed from cell suspensions by centrifugation of the cells followed by washing of the cells with buffer or culture medium. # `- F, |& G1 I( L' Q9 x' ?% c% V
我就不翻译这段话了,自己看吧。5 B. R' \# }8 g+ K
, m, _, i4 r4 B5 t( \, a. a我实验中只用过Trypsin,消化过HFF,Vero,293。没用过其他两种蛋白酶。没有更多经验可谈。 |
-
总评分: 威望 + 10
包包 + 20
查看全部评分
|