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本帖最后由 细胞海洋 于 2011-9-1 22:04 编辑
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' O# ]6 q" W3 D* v" zFundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition3 ^1 k6 I) y/ @% n) |, K9 M! F
" `0 R7 i' i0 x8 x# C$ ]+ e* o0 @In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.4 H% s0 ?" _, P6 M K" h
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Chapters Title/ O9 t/ E( v* M8 T" y
1 Introduction
: H) f( d! a1 ^& X8 } j2 Design and Equipment for the Cell Culture Laboratory
9 y- Y: ?6 {) z6 n3 Safety Aspects of Cell Culture6 u- x$ {4 k' z w
4 Sourcing of Cell Lines
' e' ]0 p4 d$ v8 K: f; Z9 J5 Cell Types & Culture Characteristics
2 v4 _6 m6 q( d6 The Cell Environment (including types of culture medium)
# r3 S8 H$ o/ y/ E% V$ i7 Cryopreservation and Storage of Cells$ |* N1 p2 ~1 k A& N* U
8 Good Cell Banking Practices
, m ` M0 k, y" N. F7 r" E2 v9 Quality Control Considerations in Cell Culture
+ f. @& ^5 t0 ?/ j y( r10 Authentication of Cell Lines2 J8 a, w* U* \$ l: {+ h
11 Alternative Culture Systems
3 e9 ^/ ]2 `: `/ G; V2 r- h& Q12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture! o3 ~( O- L& h2 X. s
2 \* S/ \2 m! dProtocols Title: \; m* t- \8 G( ]
1 Aseptic Technique and Good Cell Culture Practice) `& R" l" s" g) m+ K$ v
2 Resuscitation of Frozen Cell Lines1 \. g9 A7 U6 S4 Z l
3 Subculture of Adherent Cell Lines
* D; y }) C+ W4 Subculture of Semi-Adherent Cell Lines
. ?; }0 s! R R/ w9 S7 u" j% J W5 Subculture of Suspension Cell Lines9 n E$ z$ s9 o/ {3 Q- X
6 Cell Quantification, B& b. p* C7 t" G3 C. o
7 Cryopreservation of Cell Lines
. L0 ~; Y* u( v$ q8 Testing for Bacteria and Fungi in Cell Cultures4 Z# \. k0 r+ o- G8 m' x# @; [
9 Testing for Mycoplasma by Culture Isolation: `2 H. l4 s6 l+ l e
10 Testing for Mycoplasma by Indirect DNA Stain5 w# S: X2 ?+ E: E- s
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Glossary of Terms
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发表于 2011-9-1 15:17
