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Evidence for Human Lung Stem Cells
p* t3 Z! L, V0 S- _Jan Kajstura, Ph.D., Marcello Rota, Ph.D., Sean R. Hall, Ph.D., Toru Hosoda, M.D., Ph.D., Domenico D'Amario, M.D., Fumihiro Sanada, M.D., Hanqiao Zheng, M.D., Barbara Ogórek, Ph.D., Carlos Rondon-Clavo, M.D., João Ferreira-Martins, M.D., Alex Matsuda, M.D., Christian Arranto, M.D., Polina Goichberg, Ph.D., Giovanna Giordano, M.D., Kathleen J. Haley, M.D., Silvana Bardelli, Ph.D., Hussein Rayatzadeh, M.D., Xiaoli Liu, M.D., Ph.D., Federico Quaini, M.D., Ronglih Liao, Ph.D., Annarosa Leri, M.D., Mark A. Perrella, M.D., Joseph Loscalzo, M.D., Ph.D., and Piero Anversa, M.D.
! i( V" k$ O5 S, x! Q- M UN Engl J Med 2011; 364:1795-1806May 12, 2011
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* K; Q9 J2 i; _& S1 W( R0 x+ z: RBACKGROUND* s8 A4 }. v' |5 i1 x
" l7 e: k, A9 S# Q/ @' k; ^# [& g4 ?Although progenitor cells have been described in distinct anatomical regions of the lung, description of resident stem cells has remained elusive.
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Surgical lung-tissue specimens were studied in situ to identify and characterize human lung stem cells. We defined their phenotype and functional properties in vitro and in vivo.& `$ Z. B! b% M& v9 W
RESULTS
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5 h7 x4 j+ J% G6 o: y) q0 Y2 v4 J; t0 aHuman lungs contain undifferentiated human lung stem cells nested in niches in the distal airways. These cells are self-renewing, clonogenic, and multipotent in vitro. After injection into damaged mouse lung in vivo, human lung stem cells form human bronchioles, alveoli, and pulmonary vessels integrated structurally and functionally with the damaged organ. The formation of a chimeric lung was confirmed by detection of human transcripts for epithelial and vascular genes. In addition, the self-renewal and long-term proliferation of human lung stem cells was shown in serial-transplantation assays.* Z0 |- W8 G( }8 e* ^' y2 Q* j
CONCLUSIONS4 x, a9 D5 j0 y9 o
" C. A( @+ a: X2 G/ r3 UHuman lungs contain identifiable stem cells. In animal models, these cells participate in tissue homeostasis and regeneration. They have the undemonstrated potential to promote tissue restoration in patients with lung disease. (Funded by the National Institutes of Health.)
6 v0 n$ H% Z; X, DSupported by grants from the National Institutes of Health and Cardiocentro Ticino (to Dr. Bardelli).
* ` |5 T6 E& D$ n5 m: WDr. Anversa reports being a board member of Autologous and that a patent application has been filed by Partners HealthCare (which includes Brigham and Women's Hospital) for this class of human lung stem cells. |
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发表于 2011-5-16 09:16
发表于 2011-5-16 09:58
Thanks!