Putative Porcine Embryonic Stem Cells

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Isolation and In Vitro Characterization of Putative Porcine Embryonic Stem Cells from Cloned Embryos Treated with Trichostatin A% ~' N% L0 `0 i+ r
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We report here the establishment and characterization of putative porcine embryonic stem cell (ESC) lines derived from somatic cell nuclear transfer embryos (NT-ESCs). These cells had a similar morphology to that described previously by us for ESCs derived from in vitro produced embryos, namely, a polygonal shape, a relatively small (10–15 μm) diameter, a small cytoplasmic/nuclear ratio, a single nucleus with multiple nucleoli and multiple lipid inclusions in the cytoplasm. NT-ESCs could be passaged at least 15 times and vitrified repeatedly without changes in their morphology, karyotype, or Oct-4 and Nanog expression. These cells formed embryoid bodies and could be directed to differentiate in vitro to cell types representative of all three germ layers. Following their injection into blastocysts, these cells preferentially localized in the inner cell mass. In conclusion, we have isolated putative porcine ESCs from cloned embryos that have the potential to be used for a variety of applications including as a model for human therapeutic cloning.' P# `) ]+ I% N, h
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猪的ESC到底研究到什么程度了

jhu132llh

哎，猪的ES，最接近人类但又没有涉及到伦理道德的最好研究工具
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