脐带血来源造血干细胞的优化冻存

deron

主题：脐带血来源造血干细胞的优化冻存条件
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说明：原文来自Cryobiology，干细胞之家新闻小组成员deron翻译（转帖请注明）
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翻译内容：
9 W! V( J. C  _（本研究）优化了脐带血（UCB）来源的CD34阳性造血干细胞（HSC）的冻存条件，该方案使用程控降温（CRF）法，并应用10%乙二醇（EG）和2%DMSO的冻存液。在UCB来源的单个核细胞（MNC）冻存后，与对照组10%DMSO+2%葡聚糖-40相比，MNC回收率、CD34+细胞和总集落形成单位（CFU）均有显著提升（P<0.05）。这个研究证明新的冻存液和程控降温法与对照组的冻存液和异丙醇冷冻法（IPA）相比，具有更高的MNC、HSC和CFU回收率。因而，联合应用CRF法的这种冷冻培养基，对于优化UCB来源HSC的冻存条件以获得满意的HSC回收率，很有参考价值。: e3 n$ x' h) U+ W; x7 n: q
原摘要：The conditions for cryopreservation of CD34(+) hematopoietic stem cells (HSC) from umbilical cord blood (UCB) were optimized with a new cryo-medium containing 10% ethylene glycol (EG) and 2% dimethyl sulfoxide (Me(2)SO) using a controlled-rate freezing (CRF) method. After the cryopreservation of mononuclear cells (MNC) from UCB, recoveries of MNC, CD34(+) cells, and total colony-forming units (CFU) were significantly improved compared to those in the control cryo-medium containing 10% Me(2)SO and 2% Dextran-40 (P<0.05). This study shows that the new cryo-medium and CRF method provide better recoveries of MNC, HSC and total CFU than the control cryo-medium and isopropylalcohol freezing (IPA) method. Therefore, this cryo-medium, combined with the CRF method, is valuable for optimizing cryopreservation conditions for HSC from UCB to obtain satisfactory HSC recovery3 R- @" E0 P+ a5 A3 j

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个人理解：文章摘要短小精悍，但是里面的冻存效果检测方法值得借鉴

jhu132llh

介个' {! W- C  S3 M5 V  t
不是说DMSO对单核细胞的冻存有诱导分化副作用的吗; k8 u) X0 @! X9 r: T
文章有没有检测过这个？

cncpht

回复 deron 的帖子. J$ @, a: B0 z1 J

6 i5 t: q( x) R* Z非常感谢

hehe100

不错，很有用的东西，谢谢！