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本帖最后由 细胞海洋 于 2011-5-5 20:24 编辑 ) E. f' E$ ]% s) z+ |
; i2 D4 d1 Q* R$ ZHuman mesenchymal stem cells play a dual role on tumor cell growth in vitro and in vivo& N2 x$ J* v3 K. i4 I
Lin Li, Hui Tian, Weiming Yue, Feng Zhu, Shuhai Li, Wenjun LiArticle first published online: 19 APR 2011
' j/ I7 o, \0 v9 G* M7 C% OJournal of Cellular Physiology Volume 226, Issue 7, pages 1860–1867, July 2011
! e4 U! e9 C. H3 L% p' \0 K, \% nhttp://onlinelibrary.wiley.com/doi/10.1002/jcp.22511/full
+ Q9 I. k$ M, Q4 r5 ~6 {: HDOI: 10.1002/jcp.22511' \3 s' |4 P( _. q( e; M$ F
4 R1 g% X7 B8 Y/ E( IMSC与肺癌细胞A549或食道癌细胞Eca-109 在体外共培养过程中显示出对肿瘤细胞的抑制和诱导凋亡作用，但动物试验却得到出相反的情况——MSC促进肿瘤血管形成，提示在探索MSC做为新的治疗策略时要小心对待肿瘤病人....
. _" _; g3 ]6 C: a5 a+ B1 G; c; ]! aAbstract" S/ w% K; D/ F5 Y
Nowadays, some evidences demonstrate that human mesenchymal stem cells (hMSCs) favor tumor growth; however, others show that hMSCs can suppress tumorigenesis and tumor growth. With the indeterminateness of the effect of hMSCs on tumors, we investigated the effect of hMSCs on lung cancer cell line A549 and esophageal cancer cell line Eca-109 in vitro and in vivo. Our results revealed that hMSCs inhibited the proliferation and invasion of A549 and Eca-109 cells, arrested tumor cells in the G1 phase of the cell cycle and induced the apoptosis of tumor cells in vitro by using a co-culture system and the hMSCs-conditioned medium. However, animal study showed that hMSCs enhanced tumor formation and growth in vivo. Western blotting and immunoprecipitation data showed that the expressions of proliferating cell nuclear antigen (PCNA), Cyclin E, phospho-retinoblastoma protein (pRb), B-cell lymphoma/leukemia-2 (Bcl-2), Bcl-xL, and matrix metalloproteinase 2 (MMP-2) were downregulated and the formation of Cyclin E-cyclin-dependent kinase 2 (CDK2) complexes was inhibited in the tumor cells treated with the hMSCs-conditioned medium. According to the observation of tumor mass and the result of microvessel density (MVD), we found that the promoting role of hMSCs on tumor growth was related with the increase of tumor vessel formation. Our present study suggests that hMSCs have a contradictory effect on tumor cell growth between in vitro and in vivo, and therefore, the exploitation of hMSCs in new therapeutic strategies should be cautious under the malignant conditions. J. Cell. Physiol. 226: 1860–1867, 2011. © 2010 Wiley-Liss, Inc.; J% X* O% N- y
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发表于 2011-5-4 10:50
发表于 2011-5-4 16:39
