PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页
0 o' ^/ {0 r5 C$ l' |/ f( W
+ U2 ]1 I" E4 |9 F2 bOnline ISBN:9780470151808
! P/ [2 E* {9 Z$ ADOI: 10.1002/97804701518089 ?: K# @, e$ i5 p1 A( e
Table of Contents
+ l6 V% ~# ]% c0 G6 v! j1. Foreword
2 z5 N/ c' M" [6 Y0 A2 v- E& Q2. Preface8 c+ Y& A' P. w
3. Chapter 1 Embryonic and Extraembryonic Stem Cells
5 o9 W; f6 L& x4 R1 z1. Section A Isolation of Embryonic Stem Cells( @' v) t6 d7 Q0 ^% }
1. Introduction
* D( O+ p& ~+ X; T, \' c2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells( q! C2 A' T2 P; ]& L
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts; S- L: m# `2 A7 U1 v, f: L; z5 Z
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
5 F* g  j# d; V5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
# i1 y: _+ H* ]$ k$ }( M) e" H8 r. m% |1 c2. Section B Characterization of Embryonic Stem Cells
: F) q# Z& h; ^0 m/ g& t9 H1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
# e) x- e6 S  r) _2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and: \. `5 S, e  x. ?
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
/ A) _! X3 U5 G0 p3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
2 i6 Y9 V* T7 V8 E4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of8 V1 ]7 b! `- y( i
Pluripotency5 l) o6 D3 Z! f" s" ^; H+ i
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
# {: E0 p* @) ~4 nUsing In Vivo Biotinylation
9 k" w  U, q  v) \5 C! j0 J6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem/ ~0 J! u3 v1 L# A' ~
Cells* o3 B$ d" B+ H) Y* v% p! s. q
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional6 O2 p0 y5 a  m9 o( R
Profiling
. m- I  F( I5 x) J9 H3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells4 H2 l4 Y- D+ o& ~$ }/ B
1. Introduction
0 C+ ~# J2 w$ g4 {' K& q& W. C2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
4 ^  p2 U# j" @  e  C3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
1 l2 W- v5 F- Z8 L3 @" c4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of
) ]( a0 Q- A9 a3 n& AMouse Embryonic Feeder Layer Cells3 W' W0 W9 t+ Y
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
! J0 h$ `9 F( ^5 y. N6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic
1 E2 g6 c7 t& _Stem Cells
# Z) u! D* |% y+ J: f7. Unit 1C.6 Isolation of Human Placental Fibroblasts
+ J/ i1 k) W0 I9 E/ U4 ^8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
( j" L; h4 A1 n# ~4 ~# FStem Cells" u) Q5 Q) @8 f
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of
) G- ?. l  T9 |( q2 FROCK Inhibitor5 N3 b, R9 X) r4 N6 ^) I' o
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
: b$ i+ k0 H5 u, N11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
1 I5 s* P3 t+ S2 J3 ~  t1 y/ LStem Cells on Mouse Embryonic Fibroblast Feeder Cells
- }4 j6 s$ U* R3 n$ r12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
& j/ D9 q' X  U6 W- i1 E4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
/ B" S: y' `. Q1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap! L8 n8 Q- x0 U7 h8 B' I6 m
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
1 ~- x  s' @5 B$ f# J7 c# j) OSuspension and Hanging Drop Cultures
  o6 m* C" y1 G, q' |5 F3 a3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies- w  E1 R" V, }- a$ B
and a Description of the Hematopoietic Blast Colony Forming Assay
. r0 J+ A" l8 q8 l# W! R% m4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically6 \- ?, f8 q& e
Defined Culture Conditions- ~7 b- \1 `( g$ _' t' d$ x
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
- I. K( |) z  a' e5. Section E Extraembryonic Lineages
) a! P" S$ y' l6 w5 o9 X/ F; h. B8 Q1. Introduction4 e1 T# A$ f1 b
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation1 f8 a# Y& C9 q! o* C" n3 q% V
into Hepatocyte-Like Cells
4 I  u* ?# T: _3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta& A8 M' J, K$ f1 {. w3 y
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
* H3 Z" C! y# g6 j+ J5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells( s0 N9 }; H6 `3 s
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
% p2 |* ]! c% p* x7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
+ P6 i$ [8 |& [9 U' |9 t6. Section F Mesodermal Lineages9 u! G( U# B8 S+ `
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
1 X# t, h8 ?. n- Y9 A2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
6 J6 D2 E# Y7 ~8 h  w( U$ P9 Nwith Endoderm in Serum-Free Medium
! ?" v6 h/ C: @3 L' y/ l% ^3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells: ?: E9 e6 J) T7 @& @9 X/ B: H
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
/ N: u* \& i3 q- z1 @; q. f* U5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells9 \/ Q  ?+ e4 l5 r0 H- b, G/ e
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation
9 ?6 ~/ t2 c: H6 J' Pwith Stromal Layers
: d5 @* }% M* n' O. v4 V7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
. P, s2 R  c/ e( G, ?Murine Hematopoietic Progenitors! B! {) X3 e. o8 L  W7 H
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from6 g( C% S- \7 n
Human Embryonic Stem Cells
1 G% R! S- K) v0 P1 u5 h9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells2 @) H$ k9 V/ r
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
  j& y: R0 a- a- v: ~4 ?Progenitor Cells
4 s. u- M! e0 H. x8 s9 B7 E! i' e$ f11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
! b9 j4 P6 [! C5 u; `Hanging-Drop and Mass Culture Methods2 x8 u$ n( d0 q6 [  z/ h
7. Section G Endodermal Lineages! Y! G) G* B8 ?: E" O
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells( p3 q: c; \$ J) B
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
- H! R3 X$ W9 o" l$ m3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
, `% Z3 V$ A1 X4 C8 z, J8. Section H Ectodermal Lineages
5 s: ^5 G2 n" o8 L4 K2 C7 \; n1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
8 l. Y( D- `5 {  E2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by
4 Z8 S* a# p3 q$ B! p! x6 JTwo-Photon Microscopy8 h! S5 @' M: N) M+ l4 r% E: K: }
4. Chapter 2 Somatic Stem Cells
- W* \  z( [2 j, ?1. Section A Hematopoietic Stem Cells/ Y' E; S: q' O0 m, E4 J' f* D) @
1. Introduction
6 ^" @( }& H/ J$ Q- v8 A" m2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density4 e4 g, E: C7 S  w9 M! `" D
Gradient" a0 y8 f5 H. m
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
9 c4 k! B4 k4 [2 `; E4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
8 W1 x; E* k& y  v' D' z8 w; A5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
7 ^8 j7 O$ a7 V3 ~8 ~from Adult Mouse Bone Marrow8 f, I- c- d1 d+ W- }% a3 B
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche* s! D% o1 B* G
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
  O: B$ M- g1 B; xEmbryonic Tissues+ c% d% g) d; R- r# D: ?
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells. [" y1 q" x7 j5 l6 \  Q
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells8 Z2 b; s' u+ i+ P9 w- j6 x! p
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta% X: R( R8 c) Q) z
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
( p* C7 b; H+ |1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human. |  d7 V- a+ U! y' `, g2 \
Tissues3 }6 b; s; ~& q; {
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
/ w' ?: b' k! r5 r0 `! z; M! M* i3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells. J' W' d. n; W' v
3. Section C Cardiovascular Stem Cells; v1 j8 T  z9 p' D
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood$ w/ j& K3 i% X
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium4 ]) T' r0 @' C% q# X
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells( o+ |' m' S( v* K
4. Section D Neural Stem Cells  s: M9 S: h' c- _3 U$ \; w
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains8 t) v5 \$ O* F
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
+ {7 |3 h% V0 D% W3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and9 Q$ U% P6 D. R9 a
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
. S5 W; b9 m1 j9 P2 `. ?2 i- e: ^Forebrain Neural Stem Cells5 ^! C1 A+ _% u- A& o  T
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage' j, B9 z- \: h
Progression, and Maturation
- _. F4 e! h( M- l% j& U1 J5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
! E/ X2 u; {, D2 v7 l. QNeurons from Rodent Brains
2 q& f& n4 r5 E' a! C/ y+ k6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere  A9 N* t' s/ V7 v" S3 s3 K
Technique' D/ g* M) P# j0 [
5. Section E Germline Stem Cells
# s% d7 q: c% K: H, }& @1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila6 c6 v' K% g: _  }, s( j/ ]
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis9 R4 b' F* x" ~$ X& i( f
6. Section F Gut Stem Cells
7 Z. g% {+ A/ N9 o1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells. o- ^  ]& `( d2 Y) C
7. Section G Lung Stem Cells
! O) u. E0 \8 T& ~% j7 S- G1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
8 \1 K5 t7 T  V2 q. c( Q3 p5. Chapter 3 Cancer Stem Cells
* m5 V! _1 m# n  z" a5 h# M1. Unit 3.1 Colon Cancer Stem Cells( z  g1 c6 H, }% p2 r' @
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
, F, Q0 }, L/ K: g0 N. b3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
. \) {. |1 @4 }( R& D6 ]2 H6. Chapter 4 Manipulation of Potency
' R( X7 A& ?( l1. Section A iPS Cells  [1 s0 n/ |$ Y/ g
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming/ {8 l: \, C" t
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells6 Z# B$ ^0 L5 e" B, G$ J5 F- Q: X0 m
2. Section B Nuclear Transfer
  q3 E( G, ?! H+ f# w1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency$ F& H8 F8 i' _- n
7. Chapter 5 Genetic Manipulation of Stem Cells! }/ t* L) b. T, A) ]% _5 b- w
1. Section A Lineage Tracers in Stem Cells
) @7 X3 C" k7 A7 ~7 b& l8 J1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
. g7 r+ O% N  z7 k+ O& J2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
5 [% \0 a* q! y) d- k3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
" H9 H& [! {0 a1 ~9 _7 g/ R4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium. c/ ~( y9 e; h6 b. _
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
- J' p  \; |4 R; U, NNeocortical Neural Progenitors
1 Q% c& D5 y' R* O# P2. Section B Homologous Recombination in Stem Cells
: P$ B& q6 E6 R) ]7 ]0 d( }' m6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
/ |6 ?4 C1 i( A/ m/ `& Z3 fHomologous Recombination0 q, d* v, P: x8 D! y
8. Appendix 1 Useful Information
  J8 s8 x2 a8 ~, K7 a1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research0 y4 O6 L* E/ S; F  J
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells) J5 j' S' Y7 W; S, v
9. Appendix 2 Laboratory Equipment
( ?! J3 e' Z1 g9 `* l5 `0 J1 WStandard Laboratory Equipment9 h# E, x9 o, ]+ _) C6 M+ v
10. Appendix Suppliers
: m# s# F4 v( P. V8 ?Selected Suppliers of Reagents and Equipment
  v7 e* j& c1 _: k. _; M( I, l9 p) d, g$ u6 Y) O. T* j

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