FDA批准的个体化肿瘤免疫治疗(ZT)

marrowstem

         这是一个很有意思的治疗方法：把病人外周血拿出来，尤其是血里面的抗原递呈细胞(Antigen presenting cell）拿出体外和克隆表达出的一个重组蛋白PAP-GM-CSF一起培养。其中PAP 是前列腺癌组织里面可能表达的一个蛋白，而GM-CSF是颗粒巨嗜细胞刺激因子，一种免疫激活剂。培养的结果是想激活病人细胞的特异免疫机制。7 ^! F0 c% [8 ^( o; F, u
     这可以算是个体化治疗的一个案例吧，把个体的血细胞拿出来，经过免疫激活，再放回体内。临床研究显示用药组和对照组比，平均延长寿命四个月。临床结果不够好的原因可能是临床验证的病人群体有关。这样高度危险的治疗，一般都是病重的，晚期癌症的，有转移的才容许参与。到了那个阶段，可能再有用的药也没有回天之力了。可是这样的药物又很难在早期癌症病人身上试验。2 p0 q7 n: S8 O0 Q
    治疗整个疗程需要$93,000美金，因此每向死神讨回一天需要$775美金!  因为这个药通过了FDA, 该公司的股票价格一夜间大涨27%, 市值也膨胀到四十八亿美金。
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marrowstem

据说国内好多地方已经开展了此项研究，并有好几家医院早已在临床上做了尝试。
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tpwang

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跟一个利用iPS诱导机体产生抗HIV免疫反应的动物研究：4 d9 Z& u' c' G
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Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp160
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Shinji Yoshizaki1, Mayuko Nishi1, Asami Kondo1, Yoshitsugu Kojima1, Naoki Yamamoto2 and Akihide Ryo1*
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& I0 ?$ \5 U2 Q6 v% M• 2 Department of Microbiology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan0 k% s; k  X) E8 i  |
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• 2 Department of Microbiology, National University of Singapore, Singapore, Singapore
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# ]: C2 |4 n. G$ @Induced pluripotent stem cells (iPSCs) are artificially derived from somatic cells that have been transduced with defined reprogramming factors. A previous report has indicated the possibility of using iPSCs as an immune stimulator to generate antigen-specific immunity. In our current study, we have investigated whether human iPSCs (hiPSCs) have the ability to enhance specific immune response against a human immunodeficiency virus type 1 (HIV-1) antigen in a xenogenic mouse model. Our results show that BALB/c mice immunized with hiPSCs transduced with an adenoviral vector encoding HIV-1 gp160 exhibited prominent antigen-specific cellular immune responses. We further found that pre-treatment of hiPSCs with ionizing radiation promotes the secretion of pro-inflammatory cytokines such as interleukin-1 alpha (IL-1α), IL-12, and IL-18. These cytokines might promote the activation of antigen-presenting cells and the effective induction of cellular immunity. Our present findings thus demonstrate that a hiPSCs-based vaccine has the potential to generate cellular immunity against viral antigens such as HIV-1 gp160 in a xenogenic condition.
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% T1 b0 i* N% B! |3 l$ d- WKeywords: human induced pluripotent stem cells, ionizing radiation, pro-inflammatory cytokine, cellular immunity, vaccine
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' G! g2 i0 D8 M) A/ L: tCitation: Yoshizaki S, Nishi M, Kondo A, Kojima Y, Yamamoto N and Ryo A (2011) Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp160. Front. Microbio. 2:27. doi: 10.3389/fmicb.2011.00027

stemcell8

回复 tpwang 的帖子
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谢谢 提供文献，有问题需要请教，这里ips起到什么作用？为什么不直接用HIV-1 gp160免疫小鼠呢？请指教 这个文章时sci文章吗？有影响因子么 没查到

tpwang

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stemcell8 发表于 2011-2-28 22:36
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" b" ?1 |2 ]& A1 s+ Q1 m谢谢 提供文献，有问题需要请教，这里ips起到什么作用？为什么不直接用HIV-1 gp160免疫 ...

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6 [" t) n9 i% Y3 b/ C这是从新闻里看来的，搜了一下，网上能看到原文。http://www.frontiersin.org/virology/10.3389/fmicb.2011.00027/full9 P# Q9 b- O; @3 @) `4 ]% V# W
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据作者说，iPS作为免疫载体可以诱导比较长期的细胞免疫反应，这应该是优势之一。至于用HIV-1 gp160作实验，只是一个例子。还引了一篇湖南湘雅的相关文章：http://onlinelibrary.wiley.com/doi/10.1002/stem.234/pdf，胚胎干和iPS作为vaccination载体诱导抗colon cancer免疫反应的。
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至于这杂志，不太了解。