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lifescience1

【篇号】：1
( Y/ H3 o( V8 h【题名】：cDNA expression array analysis of gene expression in human hepatocarcinoma Hep3B cells induced by BNIPL-11 _8 {5 Y5 y* x5 C- s: ~6 F
[作者]Xie L, Qin WX, Li JJ, He XH, Shu HQ, Yao GF, Wan DF, Gu JR.7 c; c5 e3 q0 g. J; i1 }* C

* ~' ~% ^, Y0 A( u【杂志全称或缩写】：Acta Biochim Biophys Sin (Shanghai). # @3 u! K1 W- D% E) t3 C* W: P$ f
.【年份，卷（期），起止页码】：2005 Sep;37(9):618-24.+ ]. b/ t& U/ y) G" E/ b

/ M  w) l; M! U, Z' _7 j( G8 H【PMID】：16143817
( T+ a+ ?' A& D( T【全文链接】：http://www.ncbi.nlm.nih.gov/pubmed/16143817' X' }' f- t+ t" x7 m2 J
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inankai

Acta Biochim Biophys Sin (Shanghai). 2005 Sep;37(9):618-24.! i/ P. Q1 A" h6 w8 R- E6 j
cDNA expression array analysis of gene expression in human hepatocarcinoma Hep3B cells induced by BNIPL-1.
- r+ P0 ^7 r4 L+ U& y/ z) W* y# p- H2 H2 Y6 b
Xie L, Qin WX, Li JJ, He XH, Shu HQ, Yao GF, Wan DF, Gu JR.& |) {% l, h& g8 G+ T
2 d# E& a6 r* y' F* t( s5 G% \
Shanghai Medical College, Fudan University, Shanghai 200032, China.
) u- a, C: V) V$ m  H' f1 VAbstract
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. M+ i7 R& z. k) R" Z5 jBcl-2/adenovirus E1B 19 kDa interacting protein 2 like-1 (BNIPL-1) is a novel human protein identified in our laboratory, which can interact with Bcl-2 and Cdc42GAP and induce apoptosis via the BNIP-2 and Cdc42GAP homology (BCH) domain. In the present study, we established the Hep3B-Tet-on stable cell line in which expression of BNIPL-1 can be induced by doxycycline. The cell proliferation activity assay showed that the overexpression of BNIPL-1 suppresses Hep3B cell growth in vitro. The differential expression profiles of 588 known genes from BNIPL-1-transfected Hep3B-Tet-on and vector control cells were determined using the Atlas human cDNA expression array. Fifteen genes were differentially expressed between these two cell lines, among which seven genes were up-regulated and eight genes were down-regulated by BINPL-1. Furthermore, the differential expression result was confirmed by semiquantitative RT-PCR. Among these differentially expressed genes, p16INK4, IL-12, TRAIL and the lymphotoxin beta gene involved in growth suppression or cell apoptosis were up-regulated, and PTEN involved in cell proliferation was down-regulated by BNIPL-1. These results suggest that BNIPL-1 might inhibit cell growth though cell cycle arrest and/or apoptotic cell death pathway(s).