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本帖最后由 细胞海洋 于 2010-4-27 11:28 编辑
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" i- a& w$ c" u5 S! G: n内容不错，介绍也比较详细，推荐转基因动物相关的看看！2 q. a$ {& l* E+ ?' a
( a6 U7 T$ k, C) B. k) p& V
Louis-Marie Houdebine# _5 D* N8 q) O7 W- E" J1 U% B
Institut National de la Recherche Agronomique,6 S8 h3 o- x: _/ f' j/ y
Jouy en Josas, France
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Introduction ix. T! o$ h/ N+ y3 Z& d( T9 g0 Z
Abbreviations and Acronyms xiii
8 ^; `) q# Q5 _2 i! O# j& d1 From the gene to the transgenic animal 12 W1 M x5 I2 Q2 g" E' k
1.1 Genome composition 1* d2 I2 }" F) W, N
1.2 Gene structure 48 O! @; I+ N- V1 }
1.3 The number of genes in genomes 7% m2 v6 w8 y6 p
1.4 The major techniques of genetic engineering 13" v* [2 o, ?9 v6 B: y
1.4.1 Gene cloning 13; `( F$ V4 r X9 _1 ^- L F
1.4.2 DNA sequencing 14% l H. k5 r! ?8 g
1.4.3 In vitro gene amplification 14( H' E: f6 u7 k7 H9 Z* e# P
1.4.4 Gene construction 14
5 x3 D# U: v4 `- k! w e1.4.5 Gene transfer into cells 162 s) {8 g. `( c+ u& U3 p7 T# I
1.5 The systematic description of genomes 21
( U H. h- S5 R2 @1.6 Classical genetic selection 268 M& r$ W: [ k8 _' X( p: i
1.7 Experimental mutation in genomes 27& U& v- q/ E: ?- \ A
1.7.1 Chemical mutagenesis 27
" X% D0 X( a) M/ ?) e1.7.2 Mutagenesis by integration of foreign DNA 29
. U, o- K* I9 n1.7.3 Mutagenesis by transgenesis 30
+ q1 _. ]; M7 c5 n% z! L- w6 @2 Techniques for cloning and transgenesis 33
8 t: H( d3 U6 ?( d' J- [2 y2.1 Cloning 333 [; j* x6 Y; b$ a% p+ I
2.1.1 The main steps of differentiation 33
0 c: ?0 w) Z/ X7 d: O2.1.2 Cloning by nuclear transfer 37
: C) N4 W/ H) u. Z$ i4 e1 F2.2 Gene therapy 48
6 O( f, O* H8 f6 R2.2.1 The goals of gene therapy 48, b9 U) l5 x, c7 N+ I
2.2.2 The tools of gene therapy 49( ?) l" T1 z" ?1 G7 J5 U7 |
2.2.3 The applications of gene therapy 52. q5 d# m6 l* }4 r
2.3 Techniques of animal transgenesis 54
# P4 L6 z( d. c( r" X2 @( [% ^2.3.1 The aims and the concept of animal transgenesis 54- ?/ J& t" | d" X+ m. w# `4 l
2.3.2 Gene transfer into gametes 602 n9 k h9 I- \5 ~# W" ` |+ L
2.3.3 Gene transfer into embryos 65, l8 b H, w0 W; r1 g' _. T v
2.3.4 Gene transfer via cells 691 ?9 \" _5 ^+ j- [5 i0 O
2.3.5 Vectors for gene addition 73
% Z4 J& Q# b3 p# X/ g" B: P* V2.3.6 Vectors for gene replacement 85
, X- {% S8 R G9 Y: W5 d8 U5 r- k2.3.7 Vectors for the rearrangement of
" R1 v4 l Z1 e+ l. Ltargeted genes 90
1 L- F0 O9 }$ F l3 ]. t% x6 A2.3.8 Targeted integration of foreign genes 974 y0 W0 l9 v! |* E0 l( m! @
2.3.9 Non-classical vectors for the recombination
2 j4 C* Z x) u5 e* ^2 o/ tof targeted genes 105
5 h, _, t1 N- A& d2.3.10 Vectors for gene trap 106
$ s6 \3 T& w$ v* q% Q" H2.3.11 Vectors for the expression of transgenes 116" p: P3 f% v2 Q. @, K8 U
3 Applications of cloning and transgenesis 137
$ j" ]& v( n9 m3.1 Applications of animal cloning 137
# h2 v$ j7 R' d# s* Y. a p/ D3.1.1 Basic research 137
# R9 u/ j1 w$ B7 X3.1.2 Transgenesis 142
1 s1 y T3 ~" S: _$ j& `3.1.3 Animal reproduction 143
! P3 T, [7 R( t5 X/ z3 W3.1.4 Human reproduction 144+ i) O- @2 o; Q z
3.1.5 Therapeutic cloning 144
+ b) }2 h# C6 H* Z2 k7 u: {3.1.6 Xenografting 150
9 F# J' _$ ]' ]6 d7 W, p3.2 Applications of animal transgenesis 153
( N8 h9 [3 c/ @9 c+ l3.2.1 Basic research 153
1 @$ s4 _7 c) Z4 _$ a3.2.2 Study of human diseases 154) T& x, y6 Q+ S/ Y' e
3.2.3 Pharmaceutical production 159" m9 M3 A' `: W& ~, }2 u5 b. n* O
3.2.4 Xenografting 162# ?! Y, k8 d' a9 i; u# }/ p; g0 S
3.2.5 Breeding 163, E( j9 `# g5 l7 ]
4 Limits and risks of cloning, gene therapy1 d; M" D$ z0 P9 v; h
and transgenesis 171
# [$ `" _. _0 t0 o, B/ [5 B4.1 Limits and risks of cloning 173. I' F& B4 O! p9 s2 o
4.1.1 Reproductive cloning in humans 173% B& ~4 g( B+ C9 [6 M% e# p
4.1.2 Reproductive cloning in animals 1757 v- ]" W% ^# v: @+ A! Z3 M
4.1.3 Therapeutic cloning 176
- F: h: `# u% g: Z9 d! a4.2 Limits and risks of gene therapy 177) Z! C7 d$ L& C2 s) V9 S w4 j
4.3 Limits and risks of transgenesis 178
" c' v" h: K- ~# ?4 I8 X. S4 k7 P4.3.1 Technical and theoretical limits 1788 y8 D2 M# i, v5 A, P9 @
4.3.2 Biosafety problems in confined areas 179
& I. D( R& f1 q! v' C# }4.3.3 The intentional dissemination of transgenic
8 {- m6 b$ }" z: z9 ^( v9 ranimals into the environment 181
. g' l1 J0 R& S5 T6 h4.3.4 The risks for human consumers 184
0 v6 h' l. H1 }, @5 P2 z4.3.5 Transgenesis and animal welfare 185
( e4 s' y) n j6 Svi CONTENTS
7 n6 @- d X2 q* r4.3.6 Patenting of transgenic animals 187
3 U6 T: h7 m! A7 d R4 J4.3.7 Transgenesis in humans 188
+ \# n) w- d" H7 L5 K( fConclusion and Perspectives 191- K3 o6 d6 F" [6 g* ]2 M- o: \
References 199
, p0 Y, q( c8 F( VIndex 2173 v O* P: j' L" D- L ]; e0 D
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发表于 2010-4-27 11:17
发表于 2010-7-18 23:59
