（在线等待）请教各位关于Generation of TF-inducible ES cell lines

Rainnie

abbr_bff0ccfd6a54ee569e9f191ff3dec2ee.pdf (2.32 MB)

2010-4-20 13:03 上传

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; y" ^# h; ]* ^1 a2 NGeneration of TF-inducible ES cell lines
9 V1 O- G% |$ z9 J" r( A7 V$ c; \, x9 FThe exchange vector (pZhcSfi) was modified to express puromycin resistant gene and C-terminal His6-7 m/ g) O4 U: U
FLAG tag protein. For selected genes, we identified corresponding cDNA clones containing a full ORF
% T: S$ E+ U5 h2 D* Dfrom the in-house NIA mouse cDNA collection (Carter et al., 2003; Sharov et al., 2003) and NIH" h3 J; d+ c  L6 `+ Z& _
Mammalian Gene Collection (Gerhard et al., 2004). Some ORF fragments were originally obtained by
$ J" R0 c& R7 L* Z! lreverse transcription-PCR using ES cell RNAs. These full ORFs were subcloned into modified exchange
! ~; j  c7 u; [2 v* X* Uvector and verified by DNA sequencing. ES[MC1R(20)] cells carrying ROSA-TET locus were cotransfected
9 E$ V; {2 k* V0 H. n; Gwith exchange vector and pCAGGS-Cre and selected in presence of puromycin.
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原文附上
0 t5 J5 r3 ]- U- {. S因为生物基础太弱，又刚转到干细胞" d1 |4 l0 ]" w: A  S5 H
不太看懂这里面的表达谱的数据叫什么TF诱导
& V2 b5 G+ Z. [请高手不吝指教，在线等
' E& T1 ~; t. q$ Y6 _( ]感谢了
" W; [; z% n6 ?' X数据名称为“Timed induction of 50 transcription factors in ES cells reveals a common mechanism to initiate differentiations”

Rainnie

刚咨询了实验室同学，也不是很清楚，所以继续在线等

wuxiao101325

抛砖引玉：! V! k8 G: @7 |; |& D7 ]
TF：transcription factors 转录因子
3 n" f9 {! J, j  h& cTimed induction：时序性诱导' l6 B: m- X) L7 y
Timed induction of 50 transcription factors in ES cells reveals a common mechanism to initiate differentiations大意是说ES细胞中50个转录因子的时序性诱导/表达揭示其开始分化的普遍机制

Rainnie

我后来想可能差不多，就是每个TF诱导的干细胞不同时间表达的数据

iseeyou1210

good paper!!!

iseeyou1210

要是能诱导分化，然后在去分化回到ES细胞状态就强了！！

ambassador

抛砖引玉：
- n$ G, P; b- L) d$ m5 `+ E0 n) _TF：transcription factors 转录因子8 A$ G! d! f5 v" M
Timed induction：时序性诱导
( \; T  @+ H3 ?3 ]. KTimed induction of 50 t ...
5 W' s3 r6 r; ?) }; Q8 fwuxiao101325 发表于 2010-4-20 13:58

9 J2 y1 Q% E' F/ m

  u/ U, R! E8 K4 ?* j6 `2 V' e5 \8 E  Z6 A( e7 n
    “ initiate differentiations”个人觉得应该翻译成“起始分化”，即细胞如何通过时序性诱导来启动分化！

wuxiao101325

回复 7# ambassador
- A+ R2 G) [6 O/ R