你可以看看这个视频,专讲hESC在MEF上长的。/ ^0 i. |$ G- ? http://www.jove.com/index/details.stp?id=722, Y7 c# ]$ n. ~+ v0 m0 V
From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs2 p3 o& F) W; [% c. J6 `( [
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.