常用的β-actin 引物序列

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human actin f ctc cat cct ggc ctc gct gt - {* u* G' z- }- P
human actin r gct gtc acc ttc acc gtt cc 3 E$ X6 y) I, a, F4 J3 \
product size:268 . `4 m) X" O5 A0 P3 x
rabbit actin r agt gcg acg tgg aca tcc g # `. s$ ~9 z& ^- p# N8 S
rabbit actin f tgg ctc taa cag tcc gcc tag ; m$ _; f+ j2 P0 H& V! k& M
product size:295
+ r  G0 q3 x9 q+ Ymouse actin r cgt tga cat ccg taa aga cc . d) z5 E. O3 Y+ G  {
mouse actin f aac agt ccg cct aga agc ac
+ W# s8 r# [- R, F9 A6 @' wproduct size:281
1 o" y) t9 }! zrat actin f TCAGGTCATCACTATCGGCAAT - k4 m$ k6 r+ W0 |, n, n
rat actin r AAAGAAAGGGTGTAAAACGCA 1 `$ @( X' d7 `( E
product size:432 8 b, _: }7 Y/ Q% S# i& _' S
human actin r gag cta cga gct gcc tga cg
% F; Y4 G' e4 r7 v& r, Xhuman actin f cct aga agc att tgc ggt gg % E! o  \+ ?8 t* r3 B# A4 X2 D0 o
product size:416 8 G/ A; Y3 K" B* i' d
mouse actin f tca tca cta ttg gca acg agc
+ q3 a3 q9 ~' s/ j- @* Hmouse actin r aac agt ccg cct aga agc ac
6 \0 K; _/ k$ y# Mproduct size:399 ; k2 f+ N. m9 K; k( f' w
rat actin f CCCATCTATGAGGGTTACGC
5 ^' m! S8 S: ]5 L* b& jrat actin r TTTAATGTCACGCACGATTTC ! g7 l( N* u* e7 g* X# C
product size:150   M" z/ N9 O8 C7 r
rabbit actin f tct tcc agc cct cct tcc tg . k/ g7 u3 N' B7 j
rabbit actin r cgt ttc tgc gcc gtt agg t
. [3 E; @  p' `9 {& I4 Hproduct size:409
% ~5 }1 \; X; _  r内参基因名称 引物 引物最佳退火 扩增
8 N5 Q8 R& q6 A+ X2 H基因库序列号 引物名称 序列 位置 Tm 温度C 长度
, H" x, T* T9 U6 Q, U, G6 Z
/ Y1 a; D5 w# ^6 cHuman actin beta F305 ctgggacgacatggagaaaa 305-324 52.3 " i+ v+ e% c" x' ~$ S
BC002409 R868 aaggaaggctggaagagtgc 868-849 52.6 59.4 564
8 m; x  g3 X: I' _& U& KF1379 agcgagcatcccccaaagtt 1379-1398 57.3 ' `+ H* R8 P$ }; m7 b1 r: E; Y
R1663 gggcacgaaggctcatcatt 1663-1644 56.3 54 285 % R7 v+ f6 X7 Q, T1 s: `
2 a" ]4 ?/ g3 Z
Rat actin beta F18 cacccgcgagtacaaccttc 18-37 54.5 : X4 Z" s/ g& ^: g/ \
NM_031144 R224 cccatacccaccatcacacc 224-205 54.4 60.4 207
7 C, }0 f. O2 Y9 MF694 gagagggaaatcgtgcgtgac 694-714 54 8 b/ I( B5 K2 t9 n2 e
R1146 catctgctggaaggtggaca 1146-1127 53.2 57.1 452 - p/ @: A3 g! s* u7 P3 N  a$ I
8 \1 I8 y- G* b4 @$ E/ P- T  i
Mouse actin beta F91 atatcgctgcgctggtcgtc 91-110 57.5 0 o1 m8 c$ I" r8 e0 L
NM_007393 R607 aggatggcgtgagggagagc 607-588 57.8 60.4 517
: `: M2 \: H2 u! \! ~0 }9 hF1566 gtccctcaccctcccaaaag 1566-1585 54.5
$ f( ^% G8 Q. o% w$ `# y; jF1831 gctgcctcaacacctcaaccc 1831-1811 54.4 55.7 266 ; ?9 f' J2 t6 t

9 v+ F9 W0 E/ Hhuman GAPDH F369 agaaggctggggctcatttg 369-388 55.6
/ P, E- [, L1 g9 VBC004109 R626 aggggccatccacagtcttc 626-607 55.1 57.5 258
+ y0 p$ T% }* f4 \/ S6 ?- xF66 aggtcggagtcaacggatttg 66-86 52.5
& j2 n- t# E6 @" c/ u8 `R597 gtgatggcatggactgtggt 597-578 52.6 57 532
9 z/ o, ^. V2 u
5 R' X) g1 j( E4 s0 E! }3 GRat GAPDH F50 cagtgccagcctcgtctcat 50-69 55
  ~1 y- \- ?. N/ K1 G3 _) iBC059110 R644 aggggccatccacagtcttc 644-625 55.1 58.3 595 & Q& J3 b) L0 b+ r2 x* ^
F1029 acagcaacagggtggtggac 1029-1048 54.4
; x+ Y4 O/ c$ i6 n! R" Z6 uR1280 tttgagggtgcagcgaactt 1280-1261 54.8 57.7 252 . D$ Q* g; M# V3 p- J

/ G; O' \- S* a; Q+ e* TMouse GAPDH F306 aggccggtgctgagtatgtc 306-325 54 : g2 R1 q: _4 N$ d
M32599 R835 tgcctgcttcaccaccttct 835-816 54.6 58.7 530
/ Q8 ~0 w- e$ q* C/ N* n- KF49 ggtgaaggtcggtgtgaacg 49-68 55.1
3 I+ X( n) H5 y6 Q9 T4 _R281 ctcgctcctggaagatggtg 281-262 54.5 56.8 233
& y) Y, R- q5 q6 m" j. u# {
0 A4 W! j9 N3 c. K+ ORat 18S rRNA F1865 tgcggaaggatcattaacgga 1865-1886 58.6
, l, N: A& h& _# i8 c# @, C" t: a$ zM11188 R2164 agtaggagaggagcgagcgacc 2164-2143 59 59.3 300
: M3 I( G: g' j$ s* U- G
0 M! B+ s9 J# [/ l4 o8 N6 Phuman 18S rRNA F1565 cagccacccgagattgagca 1565-1584 58.3
& e1 v5 V+ Y" J4 g( @) RM10098 R1816 tagtagcgacgggcggtgtg 1816-1797 58.3 59 253
" C5 [- t. O# T0 z. q7 k  `$ L+ q( n& u4 G; U, d- G
mouse 18S rRNA F56 aggggagagcgggtaagaga 56-75 54.4
! f* k+ z  Q" i4 R7 gK013364 R296 ggacaggactaggcggaaca 296-277 54 62 241