Generation of iPS without Myc

刘流

Generation of induced pluripotent stem cells without
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, m5 P. I* e  y$ s$ ^) E7 R6 pMasato Nakagawa1,5, Michiyo Koyanagi1,5, Koji Tanabe1, Kazutoshi Takahashi1, Tomoko Ichisaka1,2,
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Direct reprogramming of somatic cells provides an opportunity to generate patient- or disease-specific pluripotent stem cells. Such induced pluripotent stem (iPS) cells were generated from mouse fibroblasts by retroviral transduction of four transcription factors: Oct3/4, Sox2, Klf4 and c-Myc1. Mouse iPS cells are indistinguishable from embryonic stem (ES) cells in many respects and produce germline-competent chimeras2–4. Reactivation of the c-Myc retrovirus, however, increases tumorigenicity in the chimeras and progeny mice, hindering clinical applications3. Here we describe a modified protocol for the generation of iPS cells that does not require the Myc retrovirus. With this protocol, we obtained significantly fewer non-iPS background cells, and the iPS cells generated were consistently of high quality. Mice derived from Myc– iPS cells did not develop tumors during the study period. The protocol also enabled efficient isolation of iPS cells without drug selection. Furthermore, we generated human iPS cells from adult dermal fibroblasts without MYC.

transposon

thanks job

托斯卡纳教父

坐等原文

juan880929

hehe

msliyv

Yamanaka在最近一次讲座中回答问题时说，cMyc引起肿瘤的概率非常高。iPS诱导干细胞培育出的小鼠一年多以后都长出了肿瘤。
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. G6 q$ d# k8 y9 f0 W3 W这篇文章是2008年的，已经不算新了

daviehe

支持