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2009-9-3 20:44:46 70
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+ K. ?% V1 F. N% [Nature Methods：人类胚胎干细胞分化的全新追踪法
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人类胚胎干细胞能分化为不同的体细胞。报告细胞系（Reporter cell lines） 能优先分化出研究着所需要的细胞类型。Nature Methods最新报道了，Elefanty和他同事发现的一种人类胚胎干细胞系-ErythRED。ErythRED受β球蛋白基因调控区序列控制。ErythRED表达仅局限于成熟的红细胞，因此可以用于检测体外红细胞分化，并确定体外胚胎干细胞转化为红细胞路径。
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2 X+ v* d# N3 y) y9 ?* X' x原文出处：, r: Z" p/ v4 F% ~8 v Q! X
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Nature Methods 6, 659 - 662 (2009) | doi:10.1038/nmeth.1364! J& V/ w) ^ Y0 d
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ErythRED, a hESC line enabling identification of erythroid cells
; F$ W% w8 a9 jTanya Hatzistavrou1, Suzanne J Micallef1, Elizabeth S Ng1, Jim Vadolas2, Edouard G Stanley1,3 & Andrew G Elefanty1,32 n: ], O9 x1 j: o
) b2 a. n7 h; e8 _2 G2 t1 MA human embryonic stem cell (hESC) line that enabled globin-expressing cells to be easily recognized would facilitate optimization of erythroid differentiation in vitro and aid in the identification of hESC-derived erythroid cells in transplanted animals. We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human -globin locus control region, is restricted to maturing erythroid cells.
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Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.
' m+ O" A9 E4 ~! F1 O! }Cell and Gene Therapy Group, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.+ Z; [/ b2 K! T9 r
These authors contributed equally to this work. |
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发表于 2009-9-8 17:04
发表于 2009-9-9 10:16
