急求如何从病人的尿液样本中分离，提取尿液上皮细胞的protocol

innatestem

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你好。谢谢你的分享，培养基的成分有哪些？你能分享一下吗
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时一清

Primary Medium： 5ml FBS，250μl P-S，100μl amphotericin B，REGM SingleQuot kit 每管 50μl，再 加入 DMEM/high glucose 和 Ham’s F12 nutrient 等量混合液加至总 50μl。 注：Store the medium in the dark at 4 °C and use it within 2 weeks. 3 S9 Z5 H2 p0 g0 N" L( v7 H
RE Proliferation Medium： 50ml REBM 培养基中加入 REGM SingleQuot kit 每管 50μl。
% m, _; I, h) d+ a: ~  I1 w( \注：Store the medium in the dark at 4 °C and use it within 2 weeks.
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Generation of human induced pluripotent stem cells from urine samples.

niliu730323143

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回复 innatestem 的帖子- M9 Z2 ~: s/ z5 X

; i: p/ d4 i  c4 B& T7 LThe;primary;urine;cells;were;then;processed;and;cultured;in;urine;cell;medium;consisting;of;a;1:1;mixture;of;DMEM/F12;culture;medium;supplemented;with;10%;of;FBS;(FBS,;PAA),;0.1;mM;non-essential;amino;acids;(NEAA),;;1;mM;GlutaMAX;(Life;Technologies),;0.1;mM;β-mercaptoethanol;and;SingleQuot;Kit;CC-4127;REGM;(Lonza).% }, _# w; `- ?+ r+ r* H$ T0 h. {
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参考文献：Generation of integration-free neural progenitor cells from cells in human urine。