PDF电子书：Cancer Cell Culture Methods and Protocols（SecondEdition）

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, i+ i& V5 V' s% p* E- C9 m# BContents
# F8 m; g% W1 c) \9 d4 KPreface.............................................................  v; ]( [6 O' Q  b+ Q4 n
Contributors.........................................................  xi
/ u3 Z7 B5 I( y6 r  G) M- O' J  1  Cancer Biology ...................................................  1
6 @0 P9 e; ?( D' I8 O2 \8 j. OIan A. Cree
2 J2 L( \% P% K- A1 U; ^* v  2  Principles of Cancer Cell Culture .....................................  13
4 Q% S# H2 J, b% K# M0 C% `Ian A. Cree 1 J' M, z. ^0 z8 {( F: \
  3  Storage of Cell Lines...............................................  27
" n! P. a; v2 r2 y8 aKatharine A. Parker
$ Y8 `, V, @" D# H+ B( W  4  Characterization and Authentication of Cancer Cell Lines: An Overview ........  35# n, O! O- p& u4 X
Yvonne A. Reid
! L. t+ E: p. Y& \' V! e: M% v% T  5  Online Verification of Human Cell Line Identity by STR DNA Typing .........  45. r5 W2 ^1 l  M9 m6 u5 L% E
Wilhelm G. Dirks and Hans G. Drexler
8 z/ J( Y4 `' P3 A" G  6  Cytogenetic Analysis of Cancer Cell Lines...............................  57/ a, A( C0 w5 m) W
Roderick A.F. MacLeod, Maren Kaufmann, and Hans G. Drexler
0 L+ s. b5 U- I# g9 O9 V  7  Cell Culture Contamination .........................................  795 K8 f, u- o  R: B$ z  p
Glyn N. Stacey & I1 F. u% g' r+ F* P( j+ r
  8  Detecting Mycoplasma Contamination in Cell Cultures  * G1 j% x7 J7 ^; T; f/ \% A1 M: a
by Polymerase Chain Reaction .......................................  93
6 B" a( K$ }5 mCord C. Uphoff and Hans G. Drexler 5 [; l4 a( a  Z! H3 b+ T
  9  Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics ........  105% i" s0 B8 h0 ^( N  p. @
Cord C. Uphoff and Hans G. Drexler " A1 A( K' D# f) b  g7 V
10  Quality Assurance and Good Laboratory Practice .........................  115
4 \* x; l9 c2 x* E' N5 W' ELouise A. Knight and Ian A. Cree
/ q, M7 I; l7 }* z11  Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning ....  125
& J( A: b/ T* T  B/ sLaura Breen, Joanne Keenan, and Martin Clynes 9 {9 \# Y$ z. w  y4 R
12  Isolation and Culture of Colon Cancer Cells and Cell Lines .................  1351 ^  {  J( c' X9 \
Sharon Glaysher and Ian A. Cree * P  \7 a# Y! u' r% w$ O- m
13  Isolation and Culture of Melanoma and Naevus Cells and Cell Lines...........  141
+ ~9 Q+ \  h* f: ?; Z7 {, }" Y6 w9 J! Z8 Z3 mJulia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett
# j1 J' Y0 F4 u5 B9 h/ J. d14  Isolation and Culture of Squamous Cell Carcinoma Lines ...................  1514 A$ ~/ U# P. \" [
Karin J. Purdie, Celine Pourreyron, and Andrew P. South ' E, d- K' O9 ^, c. \) j
15  Isolation and Culture of Ovarian Cancer Cells and Cell Lines ................  161
! {, H7 X6 T- g0 jChristian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween,  & `8 r  j3 T  H/ Q- r
Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz
* E/ @. x. ?. ?) m16  Establishment and Culture of Leukemia–Lymphoma Cell Lines ..............  181
, S: z7 K8 L- x7 o) O9 }Hans G. Drexler % X, e; {$ J+ \" L$ I$ }
17  Isolation of Inflammatory Cells from Human Tumours.....................  201, T1 @$ v8 p3 B4 e- c: c2 A
Marta E. Polak ( ~' @! _8 {* I! v3 ~* u
18  Isolation of Endothelial Cells from Human Tumors .......................  209, \2 W) ?, F! c& ~# h  M1 N
Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau,  
* Q# t& N/ ~3 `4 \9 U6 t( ~Roland S. Croner, and Michael Stürzl
" t9 O6 H8 N1 ?$ z0 u8 D* X19  Cellular Chemosensitivity Assays: An Overview ...........................  219* B% k$ o, D% F& o4 Y+ k
Venil N. Sumantran
) x7 I9 w& e; Q! ]3 ^. {20  Cell Sensitivity Assays: The MTT Assay .................................  237
; i+ o" O( M9 ^4 O' _' F3 LJohan van Meerloo, Gertjan J.L. Kaspers, and Jacqueline Cloos * r& ?; S8 y3 U
21  Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay .........  2478 j* U0 Y6 {, M6 Q
Sharon Glaysher and Ian A. Cree ( Q" c2 O% u  C# [" W  I
22  Differential Staining Cytotoxicity Assay: A Review ........................  2599 D. p; d' p% t
Larry M. Weisenthal 6 H( C) `7 l$ W, E6 h7 ~: S) e1 X
23  Real-Time Cytotoxicity Assays .......................................  285
1 ^( @. R7 o$ R' W6 r8 i' w  ^Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz,  
1 H9 @& I. W& b9 d6 |and Jonathan M. Cooper - ^  g7 _7 n( d/ W$ x, R' O9 B
24  Purification of Annexin V and Its Use in the Detection of Apoptotic Cells ......  293% l6 ~" m( k7 g( R6 y' p# O! V
Katy M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss - x3 E( O9 ~4 E* e/ S6 B
25  Measurement of DNA Damage in Individual Cells Using the Single Cell Gel , I, [& z  }9 I$ ]+ l" o) n; ~& A
Electrophoresis (Comet) Assay .......................................  309
$ C' k; W, _: W+ C6 TJanet M. Hartley, Victoria J. Spanswick, and John A. Hartley 3 \! s, d. V* k+ \% ?9 f1 G
26  Molecular Breakpoint Analysis of Chromosome Translocations  3 ^2 @; ~) `, g7 G% K& F! V
in Cancer Cell Lines by Long Distance Inverse-PCR .......................  321. D7 s( [0 p- d- R; i
Björn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod
  Z% j4 y* L" V. P8 i2 ^27  Cell Migration and Invasion Assays ....................................  3334 E+ @  n; V( G, `8 y* L! r( z
Karwan A. Moutasim, Maria L. Nystrom, and Gareth J. Thomas   C0 B$ ]0 L" y0 b  K1 }- l
28  Angiogenesis Assays ...............................................  345. |# X* Q4 _2 f7 q  m
V. Poulaki
3 B6 Y- {0 Y8 F% Y5 U2 {, n29  Flow Cytometric DNA Analysis of Human Cancers and Cell Lines ............  359
; Y4 P/ f! b0 Q7 t5 tSarah A. Krueger and George D. Wilson
# n; k1 L2 J5 B. w$ A9 E7 m# ^1 B" J30  Expression Analysis of Homeobox Genes in Leukemia/
  n3 s' ]! `6 V2 |6 q/ t# i/ sLymphoma Cell Lines .............................................  3710 f9 [( c" @7 h; [" Y
Stefan Nagel and Hans G. Drexler
, R' {7 u, S5 b1 \9 A31  Measuring Gene Expression from Cell Cultures by Quantitative  8 L+ W& b' V# w1 z9 d1 f3 L, A5 p* D
Reverse-Transcriptase Polymerase Chain Reaction.........................  381
# h1 c, X3 c( T4 ISharon Glaysher, Francis G. Gabriel, and Ian A. Cree
4 @5 v0 I, F( B( R2 p32  Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins ....  395
! `) c: W+ }# ?# S! CSamantha Larkin and Claire Aukim-Hastie
1 Y! N$ J, ~5 R. |* I; l- n- i- U33  Development of Rituximab-Resistant B-NHL Clones: An In Vitro  2 T/ q! Z* w9 M# X
Model for Studying Tumor Resistance to Monoclonal Antibody-Mediated
) r" b' y) d) O; z" r  VImmunotherapy ..................................................  407
. k; I0 h% z" T5 GAli R. Jazirehi and Benjamin Bonavida 1 c+ A' @' g5 w, a! S1 w
34  Analysis of Drug Interactions ........................................  421) G7 D6 M, e" @. _$ t
Irene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters
6 g* ~" K$ f5 o; A35  Transfection and DNA-Mediated Gene Transfer ..........................  4354 d1 K4 P. s. Y3 Y
Davide Zecchin and Federica Di Nicolantonio
0 i: i8 K: z2 y0 A; t0 aixContents
( a6 r3 `+ M# K* S: S$ G* ~36  Drug Design and Testing: Profiling of Antiproliferative  
# p6 R9 ~; }; \4 C5 T8 U7 O1 ?3 @! a2 oAgents for Cancer Therapy Using a Cell-Based Methyl-[3H]- + F+ X5 n0 Z" H$ m3 J0 n! e
Thymidine Incorporation Assay ......................................  4514 @4 J& N* b) g% |
Matthew Griffiths and Hardy Sundaram
/ `9 M% p1 y$ |8 V37  Feeder Layers: Co-culture with Nonneoplastic Cells .......................  467
0 b7 s2 O9 F5 \' q" YCeline Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South 7 f0 ^6 i5 R6 x6 A3 C2 E6 t& a
38  Xenotransplantation of Breast Cancers .................................  471
' ]4 v, u7 N6 O; YMassimiliano Cariati, Rebecca Marlow, and Gabriela Dontu
. V; n  i& ]; ~1 d6 b8 }# _Appendix A .........................................................  4838 A/ }) l2 p! j! m/ a, W
Appendix B .........................................................  487
4 `+ N. L, O5 I, @6 C# }6 gIndex ..............................................................  493

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