PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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Springer MMB Vol931 2013 共29章 552pages
* L% d; O0 H7 r) r" GCell Imaging Techniques  Methods and Protocols2 f+ W8 V5 s, Y- {8 \" N( y7 B
Second Edition+ \7 R  H; v+ N1 s$ X
Edited by
" n0 U) p2 B9 _  Q  Q% J8 ?Douglas J. Taatjes- a' ^6 N/ Q3 X9 u( e2 o% }# Y
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Contents
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! l+ N# a0 V; e4 IPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii
$ R4 g4 I) {- \; v$ w- D) RContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii' D! p# p1 `1 @/ Y+ ~5 v6 N% Y
1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       12 X, l0 m" r. k8 C" U: j! {
Douglas W. Cromey
$ T- `0 z: c  L$ j5 X2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       29
* o; X- W  t! G& M. gDonna J. Webb and Claire M. Brown  G( }/ x+ d+ x9 i( v6 q
3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   61% T* W, s+ A+ e4 z
J. Lacoste, K. Young, and Claire M. Brown* h" {2 ^2 b& k( v
4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic 9 M. P* }. @# \! R' M. i
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
" f/ s) O" v! ^) mWinifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
( k5 _! ]" U5 J3 ]! R; Y' D5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97
' ~, }& ~' u  H7 T$ jJeremy Adler and Ingela Parmryd5 F- [2 J- ?  U" N6 }3 v. i
6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    111' V" @4 n: B2 h& ]
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman( n$ k" C" e4 x" @, c* ]- n9 q. b
7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
4 ?9 J( b2 s' b6 j! PRaju Tomer, Khaled Khairy, and Philipp J. Keller2 F+ x" J5 u$ c) q  A- U+ Z
8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
" v" F+ s3 A, q$ {4 ^+ ^7 oLasse Evensen, Wolfgang Link, and James B. Lorens3 R- a( b: G# C
9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153$ A5 P. K( R% n! l  l$ K% i
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert3 J. y8 j" @" h/ I
10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
8 N8 ^. ^2 Q; q& V4 t5 W; EInterference/ V5 Q; K0 J" `& V; @/ J7 s
Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169  @& j8 \  h1 T& X+ [8 H
Wei Sun, Lehui Xiao, and Ning Fang  W6 W" h) e1 k- `
11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187: Z/ q( d) d- g7 ]! B
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
, \- H7 G; W, y12   Laser Capture Microdissection for Protein
! L4 u% E! r  e1 Q! Q# ^% ?; yand NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2138 s* Q6 b: n- S6 k: i  P4 v! C
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller," w4 T$ Q$ L; \5 T1 ^: `
Lance A. Liotta, and Virginia Espina* M. K' m: D' q# K. i; i3 k, E
13   Viewing Dynamic Interactions of Proteins and a Model Lipid& P) L1 Y- Y0 f0 D" k; s
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259( b$ T2 F6 r9 l! A" g/ b. h
Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes& N4 H+ t! \; a0 G: H- f8 W
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
* g9 C, ?; I2 ^5 j% N; H) m# XLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko: X" u; O9 t! c
15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
1 W* j7 f* l6 p  E6 K, aJoanna L. MacKay and Sanjay Kumar2 C/ p: F3 T5 x, O8 d, H% c( i  U
16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331: W0 P) t. ]4 C" r  {  d: J. A; v
Lilia A. Chtcheglova and Peter Hinterdorfer
: e  g) b. m# o) C9 E; M17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345
+ D7 }) h" j9 f# J1 J1 _Bhanu P. Jena, O: v; P! n" F) T) C5 L& Q
18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367/ T- Y  {& d/ c' e
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs
9 y' a8 E# ^! K% b19 A Novel Combined Imaging/Morphometrical Method for the Analysis* Y5 f  U. I( p9 n/ @- e
of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    391* d8 z5 x3 ^: U6 @% y9 B7 b$ W; k- Z$ U
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
1 S( V. M1 y" W" q( G2 u( @! i  u- I20   Correlative Light–Electron Microscopy as a Tool to Study- T2 ]% u- f: d, s1 \! F. \6 V
In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413/ p& w) ~- j: ?" [% d6 ]
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini1 S* r% _; U& m, Y5 |
21   Photooxidation Technology for Correlative Light- a) q, {" ^& M* j
and Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423
5 E! ^, @# u+ Z, _# j0 \Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller,
/ N( E: L- f# i9 P$ r9 o4 Q. QMargit Pavelka, and Adolf Ellinger
/ s8 q  k6 b% S' Q7 b22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  4373 m) j" d% B& e2 l& e
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and
  F  @( g: R& f% @* bMargit Pavelka; u$ B. \% w. E3 w. d8 u: l  G1 [% D
23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     449% `; ?% j& r0 X
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori" h" L( H2 _. B8 h
24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
/ i0 P6 U3 ]& T+ N# B' oNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader
( W; B( `* z) e; d3 r" k) |; ~/ u25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     483
7 T3 z8 u% @  h. {Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
4 Y6 G. c2 Q- O9 e! [6 p26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    493
2 V) l2 j. |6 D1 g- x, A3 A* HJ.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald1 m% D. f& L' U! Z. c4 [
27   Environmental Scanning Electron Microscopy Gold
) a, g% k8 R6 r3 Q0 A8 `& _Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517& q5 z9 X5 m1 f
Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi( D4 E( p, v1 y% G; r6 [8 Y
28   High-Pressure Freezing for Scanning Transmission Electron
* c& ^$ N; g/ ?' WTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     525
6 `& p+ P; J, u  R; v( H/ f; FPaul Walther, Eberhard Schmid, and Katharina Höhn
5 q& Q1 S, K8 @6 v: @7 D0 ^- ^6 c29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     5372 p9 L8 R+ ?8 S0 m/ t
Stephan Meding and Axel Walch
# X+ D6 U& y" F: Q+ S+ ZIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     5471 g6 g( u: O: t

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