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本帖最后由 细胞海洋 于 2013-1-8 09:22 编辑 9 [4 |$ C( t) h/ b! b
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( G7 \" v% O. c, L+ A: u7 e `* xSpringer MMB Vol931 2013 共29章 552pages
& d3 ~$ `$ ]: H5 sCell Imaging Techniques Methods and Protocols
3 V& P7 F6 _8 N' K( rSecond Edition
7 B3 v: k* Y9 u: sEdited by
2 F0 p! `1 k( U4 l1 C( dDouglas J. Taatjes
5 x4 m; Z6 Z4 F6 l, L! C9 |: n* u$ ?2 i$ c
' @) e4 v4 P% {' vContents
% V; W6 v/ I" z- \/ H
4 i* e7 q: l- K! x* tPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii2 T( |3 y" V! C% U
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
7 e I( D* R; K9 N6 {! [0 W X' H1 Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . . 1
0 n$ x# g$ G5 s. q1 Y DDouglas W. Cromey. \5 a! [4 t, W+ @# M6 l
2 Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 299 {1 W4 e- ]2 S2 W2 z( x
Donna J. Webb and Claire M. Brown' P1 w; ^2 C% k/ @' d
3 Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . . . . . . . . . . . . . 615 `" g1 j: s J' Q! h3 |
J. Lacoste, K. Young, and Claire M. Brown
- Q. [" W' m- x6 @4 Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic . x% k. ^, V. O1 n/ s) R$ ~; p
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
2 X- j" @) k( K' T4 QWinifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill( B! C$ C7 b, o3 z8 L
5 Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . 97
- a1 K# N& @8 O% s- `& O! VJeremy Adler and Ingela Parmryd# T$ O- R8 x7 I
6 A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . . 111* P( j- T1 \7 y; q) D+ q4 P& k, {/ _
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman# [& F2 ~! @9 _2 P' C: B# l
7 Light Sheet Microscopy in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123* H' z6 V$ L6 z; f3 g4 u
Raju Tomer, Khaled Khairy, and Philipp J. Keller, ~* b; P3 s" L# }5 x/ K1 f9 U! a
8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139+ O' N7 G- { r7 S1 h$ ~
Lasse Evensen, Wolfgang Link, and James B. Lorens
: p. {& l, a- `4 E9 Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . . 153- P$ k% T7 i% z4 I7 e/ f6 Z0 o
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
6 i9 a! Y- l3 r$ q10 Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
( d/ I: J2 a$ l2 iInterference
2 e1 W3 `& ^( w: |3 bContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . . 1694 v! M8 n5 c- J U* T' I4 N
Wei Sun, Lehui Xiao, and Ning Fang
( J5 e% [' c" `& y& |+ m# T11 Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . . 187
' h: U( _ ~. D0 fPiotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
3 }7 \' v8 Z+ T/ D1 _12 Laser Capture Microdissection for Protein+ N/ q- A: h8 u1 e7 ]
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2133 n6 } K( a- l: W
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,
% J3 P0 p' N) {; zLance A. Liotta, and Virginia Espina
7 x8 i1 o" \) V4 Q/ C4 D13 Viewing Dynamic Interactions of Proteins and a Model Lipid% j% \ r7 C/ N3 P0 K' T
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . 259' b' \: v# T) Q. w/ d
Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes
* o( t4 N, z" k4 b- Q9 y8 C14 Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .2957 V& y" k4 c5 C" j/ x/ n% e
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko# J& Q7 @6 V, J# J6 r
15 Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
7 ], x5 l6 ?3 o1 f% }7 @# c" uJoanna L. MacKay and Sanjay Kumar% J, b6 }: Z1 Q; f5 x% c4 X" o
16 Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331' R+ u$ F1 T: o& ^7 u& F
Lilia A. Chtcheglova and Peter Hinterdorfer& E: Z, C5 w$ N5 l
17 Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . . 345: K( E. l2 p0 g: t! ?
Bhanu P. Jena
7 S# s4 o6 ~" L: _; w" `' H18 Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . . 3674 Y5 i5 q; }1 {' m0 A' L* @$ @
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs6 U6 a0 O& y _, y3 t! O
19 A Novel Combined Imaging/Morphometrical Method for the Analysis
- W6 k4 p# o/ Y5 x9 U0 F" m/ aof Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . . 3917 l; i- ]+ F, D2 Q* \7 ~, h! g: u
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
- Z' @% k \8 ]/ i$ E& Y* S- T20 Correlative Light–Electron Microscopy as a Tool to Study
9 F8 t7 g" t5 t, [4 G( i9 {In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . . 413
+ ^4 u" ?& {8 |/ b! M7 zElena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
+ k% b- Q5 X; @21 Photooxidation Technology for Correlative Light9 H6 K! K8 d1 K0 k/ ?( ?$ G, w
and Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 423
/ z- B' n N2 H: HClaudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, . Y2 v n/ {7 j2 U9 }
Margit Pavelka, and Adolf Ellinger
& A! H7 {) m3 j) M5 F2 S22 Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . . 437( m. l0 _2 ~$ ]& o9 p$ T1 b4 {, e/ o
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and - @. M- A' E8 C$ k
Margit Pavelka' j. n! N2 ~6 U* b% a
23 Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4491 V1 j) }6 p5 g) o, R
Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori% [- A: q/ g* P2 D! J9 b8 S0 n4 X" r
24 Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . . 467
7 P$ |# s7 P0 t3 @4 v+ `% SNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader' K2 z- x" k' ?, O
25 Histochemical Detection of Lipid Droplets in Cultured Cells . . . . . . . . . . . . . 483
7 t4 ^+ ?; t Z: y! s+ e0 kMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
4 Z# B2 U( ~/ e8 s" }3 b8 _2 V26 Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . . 493
( A0 t" F6 a" y- s% \J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald: u" O2 k$ X$ j. `
27 Environmental Scanning Electron Microscopy Gold1 g6 J$ w) K& R# H
Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 517
% S' ]3 M6 O# J: x* v* qFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
5 |' H) K# L; W7 M28 High-Pressure Freezing for Scanning Transmission Electron# T8 `7 p! j) B
Tomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . . 525& S" Z/ x; D; J: q
Paul Walther, Eberhard Schmid, and Katharina Höhn
2 t$ x# D4 g* [! |29 MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . . 537+ ?" H7 f- ~! |# C7 F: G8 ]
Stephan Meding and Axel Walch
( t) e {! G0 g3 r9 B& E+ T! uIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547
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