Nature Methods：人类胚胎干细胞分化的全新追踪法

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2009-9-3 20:44:46 70( G9 L$ ^, q$ Y8 `; j9 i& V
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Nature Methods：人类胚胎干细胞分化的全新追踪法- w; p8 A' B- O7 r

9 ]6 |5 s1 n' u& ~人类胚胎干细胞能分化为不同的体细胞。报告细胞系（Reporter cell lines） 能优先分化出研究着所需要的细胞类型。Nature Methods最新报道了，Elefanty和他同事发现的一种人类胚胎干细胞系-ErythRED。ErythRED受β球蛋白基因调控区序列控制。ErythRED表达仅局限于成熟的红细胞，因此可以用于检测体外红细胞分化，并确定体外胚胎干细胞转化为红细胞路径。( d) G( ]7 \3 @6 t. s% \3 x
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原文出处：
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Nature Methods 6, 659 - 662 (2009) | doi:10.1038/nmeth.1364
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7 |$ T) T% d3 [0 O; b: DErythRED, a hESC line enabling identification of erythroid cells
& V5 a; Q5 ~" S9 }Tanya Hatzistavrou1, Suzanne J Micallef1, Elizabeth S Ng1, Jim Vadolas2, Edouard G Stanley1,3 & Andrew G Elefanty1,3
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A human embryonic stem cell (hESC) line that enabled globin-expressing cells to be easily recognized would facilitate optimization of erythroid differentiation in vitro and aid in the identification of hESC-derived erythroid cells in transplanted animals. We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human -globin locus control region, is restricted to maturing erythroid cells.
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0 p( \2 O$ g5 O. |8 [Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.: E' _7 J4 I8 I6 |
Cell and Gene Therapy Group, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.( x+ z/ _; y' k# B. x6 @  E5 o  T
These authors contributed equally to this work.