PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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; B, |$ o# S& Z4 M5 {  BCurrent Protocols in Stem Cell Biology 2007年完整版 1451页3 _! Y9 P1 W8 \: Z! X: E) d

' u) n/ F5 p( u) C2 POnline ISBN:9780470151808
. L) J9 C4 A  ~: PDOI: 10.1002/97804701518089 H- B. X( ^8 h& c9 I7 V$ Y
Table of Contents3 r5 r+ d. r* X5 e( h% \
1. Foreword5 a$ H& B! ^8 c; U" B% p# @
2. Preface
5 J  n! e4 c3 N' G7 M3. Chapter 1 Embryonic and Extraembryonic Stem Cells% ]/ H/ }6 ?% D3 |# K/ T: C$ {, D
1. Section A Isolation of Embryonic Stem Cells
0 S) p2 E& {5 x/ F( H1. Introduction
, G/ o! ]  P" g5 ~9 d2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
+ A9 ^) C) ?, d: o3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
4 u% C  t- ^* K: k4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells7 y0 c! ^, v& v0 Z( i. n3 s
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
4 g% g( j& Q1 G% e  S8 w; e' t1 x2. Section B Characterization of Embryonic Stem Cells
$ p) f# }3 y( z! j8 d+ u! w& M5 |1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells" V% [4 ]4 P/ b1 G3 \4 ~
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and. d) v$ W- [8 x/ Z* Q
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
  J( e$ f0 v3 I! g0 F# ]3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
! b  I" t) @+ P/ b8 {4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of
, j( E1 v( `0 Q( Y# ]Pluripotency
; [8 B$ `7 C  |# D5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells' v2 N8 c% |7 `0 ^4 w% v
Using In Vivo Biotinylation, c* k/ a0 T8 a6 k/ y; k5 w1 \
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem$ b: P& Y; l2 z5 O9 s- e2 i% X- G
Cells
+ a+ u' p  ]) n7 k5 d7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
  T& b2 ~6 x7 `, P, {( c* _9 m5 [1 r, dProfiling, s* G" T+ r8 g- t$ G2 X) B
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells# X4 }  o: |4 z( X) q
1. Introduction+ b- Z3 g5 [' S" X9 j
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro: W0 E- h0 X$ N# n
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
' t, ?, b& m' ^+ n; R# l1 O0 B0 \4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of. V3 o  M  o: d! P) e8 C
Mouse Embryonic Feeder Layer Cells
3 q5 t3 I) i+ Z& g: i5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells; B- W/ y1 c# F. h( B
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic# X. E0 H: \+ U
Stem Cells
" }1 N! R$ [: L% _9 q7. Unit 1C.6 Isolation of Human Placental Fibroblasts7 f! B$ N0 O$ l
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic" R8 [3 ^6 L* b3 v; K& {
Stem Cells
; z$ l0 \5 P+ H9 i9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of
" r2 c: A9 G' I. B% x2 cROCK Inhibitor" u. ]& T7 ~& T. t3 Q! p
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
6 Z9 y8 H( I" I2 n4 Y5 Z  u5 ?11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent9 a8 H8 V$ B$ ]1 s
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
, \0 y" J  j* J1 v12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers; G* C! e8 O0 \9 `0 f5 ?
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
* \1 ^, a( m7 T. _5 y2 n1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
% }  ]2 s  \4 |6 a! i6 @! |1 k- M2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
2 z# P4 O. T3 i, S6 Y; n' n! {0 MSuspension and Hanging Drop Cultures
% }- N* s7 j! o6 w5 P3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
) x& v! n4 M3 m% f% j  g# R, {and a Description of the Hematopoietic Blast Colony Forming Assay
! f+ K, f8 I% t4 Q4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically
* Q' |3 o9 w% k, x$ g% }Defined Culture Conditions
, P9 H* J" I" d& T1 l5 d5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells: D: F0 l/ G& t- k+ r
5. Section E Extraembryonic Lineages3 @& o" ~7 V7 p. Z* a! i
1. Introduction
# M& y$ K$ n1 a) p& v8 t; i2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation  E0 a+ d. K! |
into Hepatocyte-Like Cells
( W7 h( ^6 c( `- S* k3 W; p* x3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta* @! E* t3 t5 |
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells- y& w+ Y/ i; W# s; ^, q5 r
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells4 r4 P! F1 A; X/ }/ R7 ~! [
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells1 l# J: K& A1 c0 q4 B7 T6 v
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use! H5 X( a: r9 _0 Q% D) e2 O/ [6 ^
6. Section F Mesodermal Lineages
& T: ~; L, F6 {% A& K7 O/ p, E" Y1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells0 _) j  q. e. E5 P9 j
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
$ y! m! O, Z6 \/ L  U% cwith Endoderm in Serum-Free Medium2 C. W- W% L; u+ ?
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
' T) @6 S; v$ W1 r7 g$ x4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
' i) z9 S  j$ I% g/ k5 J% l5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
" @7 Y1 c& I) ~8 T( b6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation
9 {3 m+ y/ F) ^( V# P# ?with Stromal Layers
! ]; [+ n0 Q3 {, J! t% I  }7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
8 Y' K! J+ o+ M- o' Y" `Murine Hematopoietic Progenitors, q. f- Q; l2 u! x
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
, q: E; L2 m  c5 r* GHuman Embryonic Stem Cells2 h2 ~+ N5 p& V& E# z( x
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
1 [" ?! z; X3 ]10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac: r  U" f! w$ P$ O+ G" z% E, M
Progenitor Cells/ H) G0 X9 }& P4 |1 H# \3 Y. S
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
* n9 L4 [. d1 \. g$ H4 CHanging-Drop and Mass Culture Methods0 R  ~/ s* C2 O) S& n
7. Section G Endodermal Lineages
0 e% ~3 `5 W. G# r1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells3 L6 T5 x- @5 d
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
" q* D" H2 q' R  v6 ?3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm) Y! g& c+ E) J- e2 B" Q' T& G) S/ \
8. Section H Ectodermal Lineages
- a! `8 `2 U7 Y- e& A0 ?# U; K1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
: g! m/ @3 k1 [: Q2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by
( |) Q) I/ r. q! ATwo-Photon Microscopy6 A9 w3 s  }+ }2 z# S$ T  w
4. Chapter 2 Somatic Stem Cells
  O6 A; Z- o. Z3 {/ U+ g& L1. Section A Hematopoietic Stem Cells
7 O& j0 w9 }) n, j! Y$ d9 f1. Introduction8 ?( o' k* K4 h0 c; |" L
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density! U; s; V2 y; U1 o0 {
Gradient) M" B/ N" i8 ^  Z" Z
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
+ x! ^- X3 s  @; N" L4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood1 z+ |2 U- p( ?& i. p
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
6 u. R( M1 C# R# T5 g4 z' zfrom Adult Mouse Bone Marrow6 q/ z% l) }) H+ f* n0 C- @
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
& a8 f+ R3 G& o, U1 C) q' N' c7 o7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine
; D8 Q9 J5 s" m, V& uEmbryonic Tissues4 W6 g2 D" C3 h3 b3 p! I
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
( C  N" `: ~" o9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
# E6 G% o5 Q- u6 M; H" I10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
6 C- H# _  i( ]7 F% I1 j; \. d2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells  L/ ^& [# W4 g
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
% t, t5 S; i) N+ fTissues" F/ e! }. I: M- p+ M/ e7 A
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
. |. _& e5 _3 Y3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
. C; u' _8 b, K. I$ r. [5 Z3. Section C Cardiovascular Stem Cells
8 A4 }8 q3 Y% s+ |, y2 d' E1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood9 F# T$ X2 `+ p8 O* D' u$ @
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
  ^2 E7 `" H% T3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
3 a! B! R9 ]9 X, P2 ~3 J1 a4. Section D Neural Stem Cells
* r# {, ?7 p* ?$ r1 l3 f5 X5 u4 ]1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
, H; r5 [; N; B' |8 u# J2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
/ ^( Q$ g) w4 v  S! I$ k1 z3 d* I- r2 p0 L3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and# {( V5 N+ |: c2 d; D
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
# q) G" y/ z, }5 j1 aForebrain Neural Stem Cells
( C+ [! V) L3 _1 ?2 J, c* v4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage1 x, M+ _; r9 d! J; Q& r( @
Progression, and Maturation
6 H! ^' ?2 b0 r: Q- F) [: }1 j4 Q5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic0 M: X' d* n; R) l2 z
Neurons from Rodent Brains
1 T# m- i% V% w( K# t6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere
, L& f1 m; S9 y# }Technique+ I5 n; u( I' {: O8 V& f  @
5. Section E Germline Stem Cells4 R$ A' x- N2 m: ~7 X0 [
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila6 \! r7 r# t. N
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis: [+ Z1 l& y' ?- m
6. Section F Gut Stem Cells1 u& n7 w: I1 S* B% \  w
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
; K  d; E4 a4 l) [) J$ ^! y7. Section G Lung Stem Cells+ u- k& p3 @3 i/ z% P
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells4 G* v$ G# |$ t$ J
5. Chapter 3 Cancer Stem Cells* J, W- l( F5 F- W5 q" Q0 |
1. Unit 3.1 Colon Cancer Stem Cells: k% ^6 C; O( |3 T! j  I* ^
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model/ s# [6 O# t  p0 {
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
! G. c. `, z$ K6. Chapter 4 Manipulation of Potency
# Q/ S8 t# [% r+ ~) \1. Section A iPS Cells, R8 y9 }$ }* h$ c. S7 p
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
- P1 I8 E) i7 v; g8 e  F2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
) Q3 \2 d. g0 w( w2. Section B Nuclear Transfer
  r! N& l: Y* t: I. S) f: |0 P  f1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency8 \3 l, U+ ~) J  g% v* ^# ~
7. Chapter 5 Genetic Manipulation of Stem Cells
0 ^5 v" g+ L7 K% R1. Section A Lineage Tracers in Stem Cells
7 a6 Q0 B1 b- j$ P. J6 c3 G) v- c1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
2 }# F8 Q+ C4 g4 I/ v; i2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing4 D/ a4 ~) N0 l0 J. ?
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
8 [) F- T4 d. r5 ~, s/ H1 J4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
" @3 {% h( ?/ o$ X3 F( ^5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
+ n. s/ g. a( l& ]% xNeocortical Neural Progenitors4 B+ O) V( i& n
2. Section B Homologous Recombination in Stem Cells1 v) b+ R7 m& J6 Q; ~3 u$ R
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
/ j3 l# S! L/ Z2 k7 yHomologous Recombination
# }& D7 |- q9 |  Q( @# k) {' x- Y8. Appendix 1 Useful Information
/ u  U9 W& x9 D# a  O) q1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
; A) b# k5 v' J$ i% E* U2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells8 f9 x+ @* ?3 u5 c
9. Appendix 2 Laboratory Equipment
6 b) {3 K, f+ R9 M3 o6 U3 B, KStandard Laboratory Equipment9 r2 `, f! H) P
10. Appendix Suppliers# Z8 ^3 ~+ I, N# e0 R* X" R& l
Selected Suppliers of Reagents and Equipment
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