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abbr_bff0ccfd6a54ee569e9f191ff3dec2ee.pdf
(2.32 MB)
6 k+ ~( w' F4 J; n( _/ \Generation of TF-inducible ES cell lines
0 I* [9 Z! n0 k( g5 R# JThe exchange vector (pZhcSfi) was modified to express puromycin resistant gene and C-terminal His6-
8 e. f* |" T& U1 i7 HFLAG tag protein. For selected genes, we identified corresponding cDNA clones containing a full ORF
) D1 Y2 ^( p @% C' L4 x9 Z' @from the in-house NIA mouse cDNA collection (Carter et al., 2003; Sharov et al., 2003) and NIH
4 D6 r2 ~- u! x5 o; F0 ^3 p. cMammalian Gene Collection (Gerhard et al., 2004). Some ORF fragments were originally obtained by( n$ ~6 A# G1 N4 A- o: P, n
reverse transcription-PCR using ES cell RNAs. These full ORFs were subcloned into modified exchange" v& n, ^: `: m% `' W2 ?5 R
vector and verified by DNA sequencing. ES[MC1R(20)] cells carrying ROSA-TET locus were cotransfected
2 \9 M+ a, F5 ?/ a6 X8 Swith exchange vector and pCAGGS-Cre and selected in presence of puromycin.+ G# y: r+ {, ~& K' U
$ m3 B/ w1 ^9 `7 u" d原文附上
7 W6 ?3 L8 f, u8 c: U2 J因为生物基础太弱，又刚转到干细胞
' q' X: q# Q' d) O4 {: |不太看懂这里面的表达谱的数据叫什么TF诱导
, ]5 `" `& t8 o6 k$ O) I, b0 `; x请高手不吝指教，在线等
7 z7 [: c z/ M3 W9 }; f7 G感谢了
" S/ z! y! T4 q数据名称为“Timed induction of 50 transcription factors in ES cells reveals a common mechanism to initiate differentiations” |
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发表于 2010-4-20 13:03
发表于 2010-4-20 19:41
