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本帖最后由 细胞海洋 于 2010-8-6 08:03 编辑
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5 a, L7 C s8 w: e1 ^1 K这是2010出版的 Methods in Molecular Biology 系列 vol.621' ^0 z( g: i$ l1 |5 J
与本站朋友分享。$ r& q% Q p% h3 [% c: l
(请勿外传,以免引起不必要的版权纠纷)
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8 z9 J$ T! D' e& _) z% B1 P以下为该书前言:- M- L( I4 T- E& `5 b( B1 X( h
Although embryonic stem cells currently enjoy the public limelight and show great promise
1 U2 Z4 W' {. j! i% g2 p$ m& Nfor cell based medical therapies, it is the adult stem cells which are responsible for the
( o0 L( D8 f* w6 x1 Z9 kbody’s natural ability to fight disease, heal and recover, or fail and succumb to various
& h A+ X% I+ E7 i) [, {6 n1 v4 rmaladies. The study of mammalian adult stem cells has surged recently, most likely from a7 M5 x7 F0 e6 F4 h9 N9 @
maturation of stem cell studies in the classical developmental model organisms and in6 h! L/ Z7 B& d! N
hematopoeisis. All the tissues of the body examined so far are generated and regenerated2 A2 w: s' S4 I' V: y7 q/ t
from stem cells, it has been an important first step to adapt or devise new methods to6 w! Y5 \! U; t2 A* W) [, g: ~
identify and obtain these cells in quantity and purity for further study. Culture techniques) B2 Z7 V3 F4 @" L! L4 A$ d+ E* ?
have been optimized for managing the growth and differentiation of stem cells in vitro; as$ b# n3 D/ }/ m3 V+ n( n
some stem cells are pluripotent, often the method is to guide the fate of such cells among
1 D5 C. I7 f1 o: U2 G" X* t Hthe possible differentiation fates. Much of this work, and that in the classical model organisms,
5 T' l$ ?- \& o/ D" shas helped define the aspects of the stem cell environment or niche that are crucial% j+ h7 h: B# }5 z$ V/ r
for both growth and differentiation, and these studies have moved in vivo at increasingly
2 W. U4 |/ b% Q7 vhigher resolution. Importantly, the in vivo niche is a current target for bioengineering the
! I, L1 b8 f" Q1 l' r5 m0 tmatrix and signaling factors.( \4 B; n4 D h- K
Herein, we present methods for studying six types of mammalian stem cells, mammary,9 j( k9 P# i( H5 X+ w
neural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches
0 I. B: G+ Z' Y/ ^; fdescribed in these chapters will assist you in obtaining, characterizing, and studying these
) X# R: J% `) M1 J fcells or for adaptation to your favorite stem cells. One final note, should you find the
' Z6 r# T! s; d: ]information herein valuable and it helps you to make great discoveries in the understanding
; T0 U# G6 J1 L9 C9 s: f% Wof stem cell biology or application, by all means please let us, the authors, know! The
) d4 V- u; {. `, B _best way is of course to publish your findings, but falling short of that, please feel free to+ L* {, @ A$ U7 Z- i
ask questions or clarifications. Especially for this reason, our contact information is provided0 i. I# J; B1 E
at the end of this book.6 j0 }% [/ { b/ S0 e4 S
/ c' B- c* D# p6 {4 D附件为pdf全文 ,回复本贴就可以下载了
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