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Mayor/Elsevier
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0 B9 Y5 o E& n$ F1 x) k+ X o5 QTiny but dynamic domains define the elusive lipid raft, based on results from Madan Rao, Satyajit Mayor (National Centre for Biological Science, Bangalore, India), and colleagues. U8 K% I, J8 ~* h; z7 A
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Describing the structure and components of rafts〞membrane domains enriched in specific lipids and proteins〞has been a long-standing challenge for cell biologists. In this new report, the authors use FRET, photobleaching, and theoretical modeling to get the closest look yet at raft components called GPI-APs (GPI-anchored proteins). They show that lipid rafts contain small clusters (four or fewer molecules) of very tight-knit GPI-APs packed into an 4-nm-wide space.! h4 A- P1 x8 Y, U
: U: _4 ~7 T+ h- y" ZAbout a third of any given GPI-AP species is found in rafts. The rest remain as monomers. This percentage holds true across multiple expression levels, which is inconsistent with equilibrium-based formation. "It means rafts have to be actively maintained," says Mayor. "And within these regions, the GPI proteins form clusters."
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; x j3 z2 ` b5 I' t. ~7 G& m; DDifferent types of GPI-APs are found within a cluster, and the clusters are dynamic〞cross-linking of one species removes it from the cluster, and another species readily takes its place. The cross-linked GPI-APs formed larger groups that were endocytosed by the clathrin-mediated pathway, rather than the route responsible for uptake of raft GPI-APs. Thus, ligation of a receptor could change its fate by altering its lipid environment.7 \. [; `. a- h7 v
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Sharma, P., et al. 2004. Cell. 116:577–589.(Native clusters (black circles) reorgani) |
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发表于 2009-3-6 08:24
发表于 2015-6-18 19:26
