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本帖最后由 细胞海洋 于 2013-11-1 09:06 编辑 ) d( i% h1 C0 v9 u: O
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《神经生物学实验操作手册》目录
8 j* k5 \% Y4 S, G简介 - M0 H( W& N2 U5 u. s" }
神经干细胞及神经发育 ······································································5
- O1 O9 `5 ~2 i1 J5 F; f 神经系统疾病中的神经细胞类型 ···························································8- K, X E& w0 G$ h A
神经细胞培养及分化
: f- Y* d) V4 q# v9 V. x! k 人神经干细胞培养 ············································································ 11
) A( g Y) A' W9 i3 [6 w 大鼠胚胎神经干细胞培养 ····································································15, W2 ?/ T8 e( i, O: e
神经干细胞的无外源成分培养 ·······························································19, P& S9 q: N/ p" i: L
人多能干细胞诱导成神经干细胞 ····························································21, t6 p+ i# D3 _5 M, p! S
神经干细胞分化为神经元和神经胶质细胞 ················································31; k7 I( P7 C/ [4 _' \+ Q
神经胶质前体细胞分化为星形胶质细胞和少突胶质细胞 ·······························38
: [! u: c' s. w1 E% z 皮层星形胶质细胞的诱导和培养 ···························································41
. y* Y, r4 n. H9 d( x+ Q 皮层及海马神经元的分离、培养和鉴定 ··················································43- f" j# Y$ {9 n# Z0 s
多巴胺能神经元的诱导 (来源于人胚胎干细胞) ··········································48
$ ^1 Z6 N5 C9 N 多巴胺能神经元的诱导和培养 (来源于啮齿动物中脑) ··································56
% N$ N2 G% y& I0 n. U" c 神经干细胞的冻存 ············································································60
" F. j6 q4 r0 y' V 冻存及复苏成熟分化的神经细胞 ···························································62
" j6 Z5 e9 l/ T" t8 }* Q细胞分析
9 t" Y, o9 ^! y3 M! h 神经干细胞的细胞活性分析 ·································································64% H9 E% C4 h7 H0 H: |5 I
神经亚型特性标志 ············································································66
% k. k9 D9 H7 [; P5 B! x p 表面标志流式细胞分析 ······································································67
" ~9 I1 @$ _7 \ }5 C8 l ~. h免疫细胞化学分析 ···············································································694 s( W4 w- E$ N8 y' p; r' [
使用Fluo-4 进行细胞内钙流检测 ······························································72. H3 ]6 t. U/ |0 T& W6 a
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发表于 2013-10-18 13:08
发表于 2013-10-18 13:57
