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本帖最后由 细胞海洋 于 2010-4-19 11:09 编辑 9 O: Y5 u) D. y
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Cell culture is an invaluable tool for investigators in numerous fields. It facilitates t# u$ N2 p% f; V% a9 @
analysis of biological properties and processes that are not readily accessible at the level+ m) x- q) S$ }8 ~" i3 q
of the intact organism. Successful maintenance of cells in culture, whether primary or- j! U3 _; g( j
immortalized, requires knowledge and practice of a few essential techniques. The purpose8 ~ ?! T3 p" a1 z
of this chapter is to explain the basic principles of cell culture using the maintenance7 l7 g, r/ |) X; H: Z- l
of a nonadherent cell line, the P815 mouse mastocytoma cell line, and the isolation) `. e( p/ L" G6 h( [7 J2 _+ l
and culture of adherent primary mouse embryonic fibroblasts (MEFs) as examples.0 c. h8 O, w. b+ Q
Procedures for thawing, culture, determination of cell numbers and viability, and8 C/ j% k {# e9 {
cryopreservation are described. |
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发表于 2010-4-19 10:40
发表于 2010-5-25 10:08
