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DNA methylation dynamics in human induced pluripotent stem cells

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优秀版主 金话筒 优秀会员

发表于 2016-4-19 12:30 |显示全部帖子
DNA methylation dynamics in human induced pluripotent stem cells8 F/ y6 A* ~+ n1 `
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Abstract Indeed human induced pluripotent stem cells
0 S" w. Y* G# C(hiPSCs) are considered to be powerful tools in regenerative' S1 _" G- _. F6 L4 `* C
medicine. To enable the use of hiPSCs in the field of( Y0 \4 @" [% T) g5 J% N
regenerative medicine, it is necessary to understand the0 w  ]8 @( p$ b5 ^6 H2 R) w
mechanisms of reprogramming during the transformation4 g- P9 k1 h7 x6 W5 {$ O
of somatic cells into hiPSCs. Genome-wide epigenetic$ O8 Z# L% K+ w' t9 E
modification constitutes a critical event in the generation of
  n: ~) S7 ~  }9 v1 O8 fiPSCs. In other words, to analyze epigenetic changes in+ W, O& A( `. k
iPSCs means to elucidate reprogramming processes. We
/ N: S' o" f2 J- |' p8 W& v8 Hhave established a large number of hiPSCs derived from% L, F# Y& C$ [( d* o$ T
various human tissues and have obtained their DNA
" i& [8 G  ^9 H2 p: Kmethylation profiles. Comparison analyses indicated that
6 {$ g" g# W1 p" P+ }the epigenetic patterns of various hiPSCs, irrespective of
" ]6 K( s7 }' w! E- Ptheir source tissue, were very similar to one another and8 r: Q' A3 l/ p
were similar to those of human embryonic stem cells* B2 I' a8 x% W+ _% G1 u  m% `
(hESCs). However, the profiles of hiPSCs and hESCs" Y3 k+ k/ r# w3 p& r) ?( g
exhibited epigenetic differences, which were caused by  H5 D: m* z0 r. w; S
random aberrant hypermethylation at early passages.
7 n6 ^6 F# e0 @8 S7 pInterestingly, continuous passaging of the hiPSCs diminished4 a& k( T% R+ y
the differences between DNA methylation profiles of9 P% V2 I( @- Q5 [+ I! I% {  S6 o
hiPSCs and hESCs. The number of aberrant DNA methylation0 S9 c& q6 x: o
regions may thus represent a useful epigenetic index
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