HuES9 human ESCs are maintained on mouse embryonic fibroblast feeder as previously described (11). 2. To generate MSCs, a starting confluent 6-cm plate of HuES9 hESCs is recommended. 3. Aspirate the culture medium and rinse cells with 5 ml of PBS. 4. Add 1 ml of trypsin/EDTA and return the plate to ...
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