HuES9 human ESCs are maintained on mouse embryonic fibroblast feeder as previously described (11). 2. To generate MSCs, a starting confluent 6-cm plate of HuES9 hESCs is recommended. 3. Aspirate the culture medium and rinse cells with 5 ml of PBS. 4. Add 1 ml of trypsin/EDTA and return the plate to ...
A doctor student graduated in July 2016 need to get job opportunities Such as 1dysfunction in energy balance for body weight regulation (obesity). Regulation of dysfunction of central nervous system in regulation on metablic disorder (obesity) 2 ner ...