2014重磅炸弹来袭——Cytokine Bioassays Methods and Protocols

dahui

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9 E5 X: k7 S2 W- ICytokine Bioassays-Methods and Protocols( G! ]. U; ~0 D4 Q" r) c% O
Series: Methods in Molecular Biology, Vol. 11723 f% g, Z9 W  q
Vancurova, Ivana (Ed.)
9 q2 S  g5 |6 B2014, XIV, 367 p. 87 illus., 74 illus. in color.7 g4 _3 L6 d, E# Z4 T- K$ v1 W
A product of Humana Press
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Includes cutting-edge methods and protocols1 j- x/ U7 Z, v8 h/ q* a1 K8 B$ @; ^
Provides step-by-step detail essential for reproducible results
2 Q9 r# N% k; o9 N9 d. |Contains key notes and implementation advice from the experts* G: U1 v1 O  [8 _8 k1 ^2 ]
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Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.$ n: R  `  `0 z+ r" h

2 K9 K- @. ?7 K# B" lAuthoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.​4 N& L1 r1 w1 y% m
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dahui

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上全文啦，高清PDF版，希望对需要的人有帮忙。' [! Y1 p9 e; V
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dahui

回复 shaozi402 的帖子
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那你只能问问“海洋”了，让他帮忙处理一下。

dahui

回复 racoram 的帖子5 t% q- [# N' s

$ j& i- ]8 Z/ c+ P: s# H3 C2 _' l把你邮箱给我，发给你

dahui

回复 racoram 的帖子- `# A, i3 g& T) ?7 |. h

3 T# U' l, c/ j. R! yYangyongkun？Genscript R&D department? the world is so small。

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回复 榴榴莲 的帖子
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* q" N; Q) c" S+ X; v! e' w. V我好羡慕您呀。