着急，诱导EB能用含血清的培养基吗

south_vet

mESCs EB fomation，根据经典4-/4+方法，应该是加血清的' n! ]" }1 Y5 f5 g6 o7 S
参考Nonaka, et al. 2008. CoCl2 inhibits neural differentiation of retinoic acid-treated embryoid bodies.6 Q- D$ `6 v4 m# g
或者其他EB formation protocol ，DMEM with 10%FBS . KSR 也行，不过FBS更普遍。
- p/ \" b8 b( [" u8 W5 I6 M所以你可以放心了。