PDF电子书：RNA Purification

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这本书 是Molecular Biology Problem Solver: A Laboratory Guide系列的一个重要组成部分。因为做RNA提取和纯化，所以找了一下。这本书讲了一些关于RNA纯化的一些方法和遇到的问题该如何处理。与大家分享。0 U3 v5 G* w5 k3 l4 o
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2 L+ c% I8 i5 ]) L; K7 X5 Y) O8 G[hide][/hide]

kinase12

目录：
" Q" B, ~6 m6 aSelecting a Purification Strategy . . . . . . . . . . . . . . . .. . . . . . . . . 1986 ?+ F7 s( o& q' M
Do Your Experiments Require Total RNA or mRNA? . . . . . 198
. S6 a! W! J& w) q, qIs It Possible to Predict the Total RNA Yield from: b& G' d4 t% P+ W3 Y6 E! u
a Certain Mass of Tissue or Number of Cells? . . . . . . . . 201
$ d8 V% E; o( J5 C8 `, w% c  ZIs There Protein in Your RNA Preparation, and
0 h, {! X0 C4 t9 {, o, AIf So, Should You Be Concerned? . . . . . . . . . . . . . . . . . . . . 202( S! o) H. C1 F( o; _% l' m' d- ?
Is Your RNA Physically Intact? Does It Matter? . . . . . . . . . . 202
  S- |$ ~0 Y  Z% ?Which Total RNA Isolation Technique Is Most. O3 O: }: k* c) t
Appropriate for Your Research? . . . . . . . . . . . . . . . . . . . . . 203
: U& u& B6 L0 q6 BWhat Protocol Modifications Should Be Used for4 U4 c" I3 {4 i0 q
RNA Isolation from Difficult Tissues? . . . . . . . . . . . . . . . . 207
9 F, O$ h+ T6 M& U) rIs a One-Step or Two-Step mRNA-(poly(A) RNA)-3 _: {6 E  v. q: b" G- ^( s
Purification Strategy Most Appropriate for Your
- j/ ^  c7 r7 T, G# {* |. ?Situation? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209. b' Q2 P3 s) u* r% N+ D
How Many Rounds of Oligo(dT)–Cellulose. [! A% X: }+ f) d
Purification Are Required? . . . . . . . . . . . . . . . . . . . . . . . . . 2107 _3 t0 C; d$ G7 k" g2 T
Which Oligo(dT)–Cellulose Format Is Most7 o4 E( r; ]4 r0 i
Appropriate? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210
' ?0 R- A0 K; y' i1 H1 D) h5 q9 ZCan Oligo(dT)–Cellulose Be Regenerated and Reused? . . . 211
1 F( H  d0 q9 H3 cCan a Kit Designed to Isolate mRNA Directly from% s9 ^$ ^+ \3 b1 {
the Biological Sample Purify mRNA from Total RNA? . . . 212
* V/ r$ z  T6 IMaximizing the Yield and Quality of an RNA Preparation . . . 212
4 i1 o$ A" U: j7 \What Constitutes “RNase-Free Technique”? . . . . . . . . . . . . 212' i* o5 g2 g  j1 I6 O" m
How Does DEPC Inhibit RNase? . . . . . . . . . . . . . . . . . . . . . . 2130 [4 F) {' |7 j& d) P- z# \
How Are DEPC-Treated Solutions Prepared? Is
( d3 H0 p. p6 o0 k. eMore DEPC Better? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
" [. q, V! I- l% r  Z& _4 wShould You Prepare Reagents with DEPC-Treated Water,& b7 y1 n" m& F, }3 a1 p
or Should You Treat Your Pre-made Reagents with
+ A  J/ F- F, P. A$ q! L, FDEPC? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2144 K9 a. K4 A3 I! e7 F2 h8 B
How Do You Minimize RNA Degradation during Sample+ {0 J- l  G5 o
Collection and Storage? . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2149 ?" G& l; g, s$ @) s/ ^$ N
How Do You Minimize RNA Degradation during Sample
, L- B  c( w6 E! f/ JDisruption? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215
. I+ }! h& J" W1 a  E3 TIs There a Safe Place to Pause during an RNA8 h+ i! C& h: x: g* g  N, G, C# x
Purification Procedure? . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2187 G3 ^. }5 u" U2 P4 @# P) w1 b4 h% _
What Are the Options to Quantitate Dilute RNA
' j1 a6 e# ^) H- f& M) \Solutions? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2181 U' F+ I4 R/ J# n) a, \
What Are the Options for Storage of Purified RNA? . . . . . . . 219
: F1 f5 J; ], _$ T* ^Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 220' D) ~- L; h4 L0 F% p- I
A Pellet of Precipitation RNA Is Not Seen at the End of
2 M4 B, m5 D4 o0 ^, L0 cthe RNA Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 220
  }& H% M) L) Q7 G) O3 w7 aA Pellet Was Generated, but the Spectrophotometer
! X( z# U& O1 D$ s% pReported a Lower Reading Than Expected, or Zero
( V& p. s" f2 ~6 h+ ZAbsorbance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221
; v) H3 Q. @7 i! z5 b1 ^' h. DRNA Was Prepared in Large Quantity, but it Failed$ W8 |, Y. K6 ?+ p  A" Q# o
in a Downstream Reaction: RT PCR is an0 S9 [9 @! J- |( P) _3 s! ~
Example . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221
  ]2 x; S4 r  XMy Total RNA Appeared as a Smear in an Ethidum3 p/ a/ B! R; D; a1 [, z) p
Bromide-stained Denaturing Agarose Gel; 18S and
  Z* t* K1 B  r! H, X# A28S RNA Bands Were not Observed . . . . . . . . . . . . . . . . 222* Y  @6 I3 j3 t
Only a Fraction of the Original RNA Stored at -70°C9 D1 {! s5 o, D2 U1 q
Remained after Storage for Six Months . . . . . . . . . . . . . . 222
+ \2 H/ @) w: H6 K) M+ \8 n8 NBibliography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 222

jhu132llh

thanks

tjbone

先谢谢了！！！！！

yourwish140

看看

hormone008

来看看！

gyuxutong

谢谢，正在做RNA提取和纯化，参考下

daviddow

xiexie fen xiang

doctor009

自从把我搞成新手后什么东西也看不到了！

军医

谢谢分享