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1. 纪家葵 教授、博士生导师
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http://www.tsinghua.edu.cn/publi ... 4800323161080_.html
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1992-1997 美国爱荷华州立大学(生物化学系) 学士与硕士
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1997-2003 美国康奈尔大学医学院/史隆凯特林癌症研究中心 (分子、细胞生物与遗传学系)博士
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2004-2007 美国加州大学旧金山分校博士后2 ^$ A6 u2 s8 Y) ]2 u. v& Y
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2007-2010 美国斯坦福大学 副研究员* A0 J. b( v# ^- W9 m# Z+ ^
9 l" [* g. g7 o/ ]$ E* }2010-至今 清华大学 教授8 _+ u2 m: ` T
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研究方向
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; P) e# r' f$ Y- s8 M4 @ 人类干细胞及生殖细胞的分化机制: 利用人类干细胞定向分化技术，结合分子生物学及细胞生物学，研究生殖细胞的分化机理，建立体外分化精子、卵子的系统平台( w6 w8 A" I. a9 S( ]
8 j" Y0 t& w8 N5 F1 a, ] Y 人类遗传疾病的研究与治疗: 结合细胞重编程及定点基因重组技术, 研发治疗遗传疾病的途径& S) I; ~1 k5 \+ i3 P5 T. V
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Publications8 ~6 }! a. O" z2 K
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1.Ha Nam Nguyen, Blake Byers, Branden Cord, Aleksandr Shcheglovitov, James Byrne, Prachi Gujar, Kehkooi Kee, Birgitt Schüle, Ricardo E.Dolmetsch, William Langston, Theo Palmer, Renee Reijo Pera. LRRK2 Mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress. Cell Stem Cell. 2011 Mar 4; 8(3):267-80.
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2.Sarita Panula, Jose V. Medrano, Kehkooi Kee, Rosita Bergström, Ha Nam Nguyen, Blake Byers, Kitchener D. Wilson, Joseph C. Wu, Carlos Simon, Outi Hovatta, & Renee A. Reijo Pera. Human germ cell differentiation from fetal- and adult-derived induced pluripotent stem cells. Human Molecular Genetics, 2011 Feb 15, 20(4):752–762.
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: m8 @, T8 A- a% Y& N! M0 |3.Kehkooi Kee*,#, Martha Flores*, Marcelle I Cedars, Renee A Reijo Pera#. Human primordial germ cell formation is diminished by exposure to environmental toxicants acting through the AHR signaling pathway. Toxicological Sciences. 2010 Jun 18. [*:Contributed equally; #: Corresponding authors]
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4.Kehkooi Kee, Renee Reijo Pera, Paul Turek. Testicular Germline Stem Cell. Nature Review of Urology. 2010 Feb;7(2):94-100.: N* }/ O7 I! a' }+ q j. {
, q6 K5 v7 g; m2 m+ Z. n5.Kehkooi Kee, Vanessa Angeles, Marty Flores, Ha Nam Nguyen & Renee Reijo Pera. DAZL, DAZ, and BOULE modulate human primordial germ cell and haploid gamete formation. Nature. 2009 Nov 12; 462(7270):222-5.
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6.International Stem Cell Initiative members. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature Biotechnol. 2007 Jul; 25(7):803-16.
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- \9 I4 p8 `0 r. F4 e3 H7.Kehkooi Kee, Joanna Gonsalves, and Renee Reijo Pera. Bone morphogenetic proteins induce germ cell differentiation from human embryonic stem cells. Stem cell and Development. 2006 Dec; 15(6):831-37.' ^$ H! ~6 E* W& B
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8.Kiersten A. Henderson, Kehkooi Kee, Shohreh Maleki, Paul Santini, and Scott Keeney. Cyclin-dependent kinase directly regulates initiation of meiotic recombination. Cell. 2006 Jun 30; 125(7):1321-32.
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- e9 l o$ q& o9.Kehkooi Kee, Reine U. Protacio, Charanjit Arora and Scott Keeney. Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes. EMBO J. 2004 Apr 21;23(8):1815-24.: r7 Y# q* F; L9 v8 j% F
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10.Charanjit Arora, Kehkooi Kee, Shohreh Maleki, and Scott Keeney. Antiviral protein Ski8 is a direct partner of Spo11 in meiotic double-strand break formation, independent of its cytoplasmic role in RNA metabolism. Molecular Cell, 2004 Feb 27;13(4):549-59.
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% R' o6 }& B- @. N( H11.Kehkooi Kee and Scott Keeney. Functional Interactions Between SPO11 and REC102 During Initiation of Meiotic Recombination in Saccharomyces cerevisiae. Genetics. 2002 Jan;160(1):111-22.0 ?0 ^: Q5 d8 D8 U, o
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12.Kehkooi Kee, Luming Niu, and Eric Henderson. A Tetrahymena thermophila G4-DNA binding protein with dihydrolipoamide dehydrogenase activity. Biochemistry. 1998 Mar 24;37(12):4224-34.& O5 S, @' p4 |
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Contact info:
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Tel:+86-10-62794200(office) , 62785820 (lab)+ @- ~, c1 n, N1 p, C+ @
0 e# z ]; ]/ ^; R( [9 ~/ _ F# |Email: kkee@tsinghua.edu.cn
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9 G( C) z5 f2 C C" b3 E5 ?- p+ W2. 沈晓骅 教授、博士生导师
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" ~6 q) H( P1 z, Z教育经历 EDUCATION
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5 {9 [- a, m( e- E: B4 O2003 博士, 美国密歇根大学,医学院生物化学系 Ph.D. Biological Chemistry, University of Michigan, USA
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1996 学士, 南开大学,生命科学院生物化学系 B.S. Biochemistry, Nankai University, Tianjin, China( J8 A5 q9 a9 X) l; H9 C
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2 a/ [' x5 C2 r) v- f+ t% ?2010 教授， 清华大学 Professor, Tsinghua University, Z$ ]( [2 s/ p6 o( w2 Q" I
" e9 G% v6 ?; z4 |2009 博士后讲师，美国哈佛大学医学院, Dana-Farber 癌症研究所, 波士顿儿童医院: ^# x; o6 T: D' g
. B+ X( f$ X/ q' c! JInstructor at Dana-Farber Cancer Institute, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA: k! d; P2 s/ J
0 f1 W# C% u% s" r( ~* o$ o$ v2004 博士后研究员，美国哈佛大学医学院, Dana-Farber 癌症研究所, 波士顿儿童医院
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* k+ p% C* T% _' n) R9 G% \( Q, X% BResearch fellow at Dana-Farber Cancer Institute, Children’s Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA( J* H" S' y# c; a
2 s6 K) ?1 Y) \. ^7 t# |8 V1 a s2003 博士后研究员，美国密歇根大学 4 @- C: a. d% o! g0 i
HHMI associate at Howard Hughes Medical Institute (HHMI), University of Michigan, USA 4 e/ {- u4 e, \2 X' l
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科研概述
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/ f( k' i' c, O, _: w 研究方向为干细胞生物学和表观遗传学。胚胎干细胞具有形成人和动物个体所有类型细胞的多向分化潜能， 并可以不断自我更新。胚胎干细胞的这两个特性使其不仅在生物学研究上有重要意义，而且对其用于细胞代替疗法，有不可估计的医疗用途和前景。高等生物是由多种类型的细胞组成的。它们拥有一样的遗传物质， 但是不同的表观遗传信息。生物体发育的过程就是在不同细胞中建立多种表观遗传信息的过程。生命科学领域最重要的研究问题包括解析各类细胞特有的表观遗传信息，及探索它们是如何被建立，如何在不同的细胞中选择性地影响部分基因表达，从而决定细胞功能和命运。表观遗传机制的突变常和人类疾病紧密相联。- N* W) k% K# W
- ~& ~/ ^0 W$ R% R 我们主要运用跨学科生物手段，包括小鼠遗传学，分子及细胞生物学和基因组及疍白组学等系统生物学技术，研究表观遗传学机理在干细胞分化，细胞功能和命运决定中的作用。研究成果将会提供重要的框架在系统和分子水平上认识表观遗传学机理在调控基因表达，干细胞分化、发育和疾病中的作用，促进干细胞疗法的临床实现，并提供新颖的药物线索治疗癌症，为未来人造细胞的构建提供重要的设计蓝图。 5 T. r5 x7 f" D& Z8 ~
$ H: h7 g) ^: q+ h5 \" aRESEARCH SUMMARY
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7 ]$ B3 u" B: F+ W7 r& T) D- h3 ? Our research is aimed at understanding the role of chromatin structure in influencing gene expression and cell fate specification. Stem cells are remarkable cells as they possess ability to self-renew and differentiate. Importantly, cancer cells often acquire stem cell-like gene expression programs and unlimited self-renewal. Stem cell differentiation is accompanied by epigenetic changes. As all cells in the body contain the same or very similar set of epigenetic machinery, one intriguing question is how cell specific epigenetic landscapes are established. The major portion of our research is to elucidate how epigenetic mechanisms are guided by and interact with lineage-specific factors, including proteins and/or non-coding ncRNAs to define cellular states in development and disease.
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Polycomb group proteins (PcG) mediate epigenetic regulation through trimethylation on histone H3 lysine 27 (H3K27me3). An initial, yet critical step in PcG regulation of gene expression is the targeting of a protein complex named Polycomb repressive complex 2 (PRC2) to specific regions of chromatin to establish the repressive H3K27me3 mark. Despite the essential role for PRC2 and H3K27me3 in execution of stem cell pluripotency and in development, little is known about how PRC2 is regulated and targeted to the chromatin and how the H3K27me3 mark is maintained and reestablished during cell division and fate transition.
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We use state-of-art, high-throughput and integrative technologies across proteomic and genomic systems, together with mouse genetics, biochemical, molecular and cell biology tools to investigate the recruitment and regulation of PRC2 in stem and cancer cells. We aim to obtain a comprehensive and dynamic view of how transcription factors, ncRNAs and DNA motifs collaborate in targeting and regulating PRC2 during stem cell differentiation, and how disregulation in the above processes leads to human disease. Knowledge in PRC2 regulation and function will provide insights on how other epigenetic marking systems interact with tissue-specific factors in cell fate determination. Systematic investigation of epigenetic events during stem cell differentiation will increase our understanding of how stem cell programs are maintained and executed, assist the prediction of epigenetic changes in other developmental contexts and pathological conditions, and suggest innovative approaches to manipulate cell fates, facilitating realization of the therapeutic potential of stem cells. + @; k5 e: w t! k# W: x( J
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发表论文、著作 PUBLICATIONS
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7 Z, a* g: q( t9 W I- U ^1 、 Wilson BG, Wang X, Shen X, McKenna ES, Lemieux ME, Cho Y, Koellhoffer EC, Pomeroy SL, Orkin SH, Roberts CWM. Epigenetic antagonism between Polycomb and SWI/SNF complexes during oncogenic transformation. Cancer Cell 2010In press.
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2 、 Shen X, Kim W, Fujiwara Y, Simon MD, Liu Y, Mysliwiec MR, Yuan G, Lee Y, Orkin SH. Jumonji modulates Polycomb activity and self-renewal versus differentiation of stem cells. Cell 2009; 139(7): 1303-1314.
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3、Shen X, Orkin SH. Glimpses of the epigenetic landscape. Cell Stem Cell 2009; 4(1): 1-2.
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4 、Shen X, Liu Y, Hsu Y, Fujiwara Y, Kim J, Mao X, Yuan G, and Orkin SH. EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol Cell 2008; 32(4):491-502.
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5 、Kim J, Chu J, Shen X, Wang J, Orkin SH. An extended transcriptional network for pluripotency of embryonic stem cells. Cell 2008; 132(6): 1049-61.
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6 、Orkin SH, Wang J, Kim J, Chu J, Rao S, Theunissen TW, Shen X, Levasseur DN. The transcriptional network controlling pluripotency in ES cells. Cold Spring Harb Symp Quant Biol. 2008；73: 195-202.
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: B4 f) h2 ?) e( r9 H; ?4 _: s. o7 、 Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH. A protein interaction network for pluripotency of embryonic stem cells. Nature 2006; 444(7117):364-8.
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8 、 Zhang K, Shen X, Wu J, Sakaki K, Saunders T, Rutkowski DT, Back SH, Kaufman RJ. Stress in the endoplasmic reticulum activates s1p- and s2p-mediated cleavage of CREBh, a novel liver-specific transcription factor required to induce the acute phase response. Cell 2006; 124(3): 587-99.
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, }* H, W9 @: v3 A& ^9 、Shen X, Ellis RE, Sakaki K and Kaufman RJ. 2005. Genetic interactions due to constitutive and inducible gene regulation mediated by the unfolded protein response in C. elegans. PLoS Genetics. 2005; 1(3):e37。
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10 、Shen X, Zhang K, and Kaufman RJ. The unfolded protein response - a stress signaling pathway of the endoplasmic reticulum. J Chem Neuroanat.2004; 28(1-2):79-92." W* b3 u8 P7 m0 M
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11 、Lee K, Tirasophon W, Shen X, Michalak M, Prywes R, Okada T, Yoshida H, Mori K, and Kaufman RJ. IRE1-mediated unconventional mRNA splicing and S2P-mediated ATF6 cleavage merge to regulate XBP1 in signaling the unfolded protein response. Genes Dev. 2002; 16: 452-466.
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9 y' n* Z: k* p# _12 、Kaufman RJ, Scheuner D, Schroder M, Shen X, Lee K, Liu C and Arnold SM. A role for the unfolded protein response in nutrient sensing and differentiation. Nat Rev Mol Cell Biol. 2002; 3(6):411-21.
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13 、Shen X, Ellis RE, Lee K, Liu C, Yang K, Solomon A, Yoshida H, Morimoto R, Kurnit DM, Mori K, and Kaufman RJ. Complementary signaling pathways regulate the unfolded protein response and are required for C. elegans development. Cell 2001; 107: 893-903.1 H! Y+ U% f) n1 f5 s, a2 d
+ @3 g( x3 c% E3 @3 ?4 Y p9 q14 、Norris SR, Shen X, and DellaPenna D. Complementation of the Arabidopsis pds1 mutation with the gene encoding p-Hydroxyphenylpyruvate Dioxygenase. Plant Physiol. 1998; 117(4): 1317 –1323." n6 q9 ]+ ]6 j. g' d
: P$ }% Y Q; n4 a15 、Chen Y and Shen X. 1998. Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction. Chinese Journal of Birth Health & Heredity 1998; 6(4): 10 – 13.
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3 n; K8 a; Y7 h# d# ^+ c) |Email: xshen@tsinghua.edu.cn/ k- A* H& @# b' D
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3. 沈沁 教授、博士生导师
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http://www.tsinghua.edu.cn/publi ... 1305523644041_.html: N1 X6 o' r- u9 S5 O) S$ m2 W5 @. p
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教育经历 1 U/ ~- q8 }) ], U& r/ c( p8 m
4 Z$ p! m L: T# _6 t. }3 w. LEDUCATION* T* I/ v, ^8 B" ~
9 J) y& z5 v y& A) Y7 u2001年 博士PhD, Albany Medical College, Neuropharmacology and Neuroscience
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1999年 硕士MS, Albany Medical College, Neuropharmacology and Neuroscience 4 r$ C! @8 K5 i0 [, J* G7 Z
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1991年 学士 BS, 上海医科大学药学院药理专业
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工作经历 . @" }: l2 J- s
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2010 教授， 清华大学
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2007 助理教授, 纽约神经干细胞研究所
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2006 助理教授, 美国Albany医学院神经药理和神经科学中心. |: m( Z2 e1 m# H
5 g2 q9 ~+ m) U7 U8 Y2 ]2001-2006年 博士后研究员，美国Albany医学院神经药理和神经科学中心& m5 u( q3 e8 H0 |6 I& O: @# c
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1991-1996年 助教和助理研究员, 上海医科大学(现复旦大学上海医学院)神经生物教研组, 医学神经生物学国家重点实验室 5 e- h, B, @+ [& T! _
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' S% b p5 }7 W4 V0 u 将利用细胞生物学, 分子生物学以及活体细胞和组织影像技术, 结合多种体内和体外神经干细胞研究模型，研究神经干细胞在正常脑内的的发生和发育及其与微环境的相互影响, 神经干细胞自我更新和分化潜能的分子机理, 以及神经干细胞在发育性神经系统疾病和神经系统损伤疾病的再生修复功能, 并研究神经干细胞和血管内皮细胞相互调控在脑肿瘤形成中的作用. 实验室还将以人体ES细胞和IPS细胞神经诱导的模型研究形成各种神经细胞的最佳细胞培养方案和神经系统疾病的发生机制.
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发表的论文、著作- l. j x' M6 F' r2 L# C6 |
* y7 C2 ?0 Q. ?, ]; ~1. Erzsebet, K., Goderie, S., Wang, Y., Lin, G., Roysam, B., Shen, Q., Temple, S. (2010) Adult SVZ lineage cells home to and leave the niche via differential responses to SDF1/CXCR4 signaling. Cell Stem Cell 7(2):163-73. H9 G) ]& P' e( i8 @
y* E3 `9 d. `5 p8 z! ?1 a7 {2. Shen, Q. and Temple, S. (2009) Fine control: microRNA regulates adult neurogenesis. Nature Neurosci. 12 (4): 369-370 8 Q0 D+ b/ N0 O2 |- m+ m8 {
* ^& D0 }) X6 a% o3. Slater, J.L., Landman, K.A., Hughes, B.D., Shen, Q., Temple, S. (2009) Cell lineage tree models of neurogenesis. J Theor Biol. 256(2):164-79. Epub 2008 Oct 15.% T# T% U, w# ]7 ~; ?8 E
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4. Kokovay, E., Shen, Q., Temple, S. (2008) The incredible elastic brain: how neural stem cells expand our minds. Neuron 60(3):420-9. Cited by 99 i) F3 C1 }% b7 s7 ^9 c
: K/ d) n1 R- b S! F: f5. Shen, Q., Wang, Y., Kokovay, E., Lin, G., Chuang, S.M., Goderie, S.K., Roysam, B., Temple, S. (2008) Adult SVZ stem cells lie in a vascular niche: a quantitative analysis of niche cell-cell interactions. Cell Stem Cell 3(3):289-300. ( }3 r o- K+ X5 U
/ T3 }) p' h1 T$ ~' J- X! v- k; R6. Shen, Q., Wang, Y., Dimos, J.T., Fasano, C.A., Phoenix, T.N., Lemischka, I.R., Ivanova, N.B., Stifani, S., Morrisey, E.E., Temple, S. (2006) The timing of cortical neurogenesis is encoded within lineages of individual progenitor cells. Nature Neurosci. 9(6):743-51 (corresponding author)
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7. Al-Kofahi, O., Radke, R.J., Goderie, S.K., Shen, Q., Temple, S., Roysam, B. (2006) Automated cell lineage construction: a rapid method to analyze clonal development established with murine neural progenitor cells. Cell Cycle 5(3):327-35.
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8. Shen, Q., Goderie, S., Jin, L., Karanth, N., Sun, Y., Abramova, N., Vincent, P., Pumiglia, K., Temple, S. (2004) Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells. Science 304:1338-1340 3 j7 g7 L8 g5 y7 C
' D, m/ S+ v: z' C1 D( R9. Li, H.S., Wang, D., Shen, Q., Schonemann, M.D., Gorski, J.A., Jones, K.R., Temple, S., Jan, L.Y., Jan, Y.N. (2003) Inactivation of Numb and Numblike in embryonic dorsal forebrain impairs neurogenesis and disrupts cortical morphogenesis. Neuron 40(6):1105-18.
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, v9 c i+ l/ H10. Shen, Q. and Temple, S. (2002) Creating asymmetric cell divisions by skewing endocytosis. Sci STKE. (162) E52.
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4 P) D. k# r; J) ~2 ]' |2 h2 l- h11. Shen, Q., Zhong, W., Jan, Y.N., and Temple, S. (2002) Asymmetric m-Numb distribution is critical for asymmetric cell division of mouse cerebral cortical progenitor cells. Development 129:4843-4853. 0 z' Z- d9 r4 ^! W7 v/ M( E
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12. Atluri, P., Fleck, M.W., Shen, Q., Mah, S.J., Stadfelt, D., Barnes, W., Goderie, S.K., Temple, S., Schneider, A.S. (2001) Functional Nicotinic acetylcholine receptor expression in stem and progenitor cells of early embryonic mouse cerebral cortex. Dev. Biol. 240(1):143-56. cited by 35+ s" P6 y% B9 A7 w/ b
! }# `9 t- C5 M) {0 i0 {1 ^+ O13. Qian, X., Shen, Q.*, Goderie, S., He, W., Capela, A.M., and Temple, S. (2000) Timing of CNS cell generation: a programmed sequence of neuron and glial cell production from isolated murine cortical cells. Neuron 28:69-80. * co-first author
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14. Qian, X., Goderie, S., Shen, Q., Stern, J. H. and Temple, S. (1998) Intrinsic programs of patterned cell lineages in isolated vertebrate CNS ventricular zone cells. Development 125:3143-3152 P- _7 z2 P* V5 i+ t. H# x% b7 x
+ K! b/ U: F& a3 E& n0 k15. Shen, Q., Qian, X., Capela, A. and Temple, S. (1998) Stem cells in the embryonic cerebral cortex: Their role in histogenesis and patterning. J. of Neurobio. 36:162-174
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16. Shen, Q., Ni, W., Miao, B., Gu, Y., Xu, R., and Lu, R. (1994) Purification and Characterization of Cholecystokinin Binding Protein from Human Brain Cortex. ACTA ACADEMAIE MEDINAE SHANGHAI 21:31-34
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' @% L2 O2 Y- z17. Shen, Q. and Lu, R. (1992) Progress in the Study of Cholecystokinin and its Receptors. Chemistry of Life 12:27-288 U. i/ l0 v" Q5 X
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联系地址： 100084 ，清华大学医学院
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TEL： 86-10-627980874 u7 b" W6 t4 w9 I5 ]* D9 `! j7 `
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E-mail： shenqin@mail.tsinghua.edu.cn 1 r, O$ {. E: X) u% P
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4. 郭伟 教授、博士生导师
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http://www.tsinghua.edu.cn/publi ... 4337095515740_.html0 S2 j i1 f& ]! A1 E; K* g
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2010-至今 清华大学教授' U, U* T9 V/ q; J" Q3 |7 t
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2003-2010 美国加州大学洛杉矶分校（UCLA）博士后
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2003 得克萨斯大学M.D. 安德森癌症研究中心（M.D. Anderson Cancer Center）博士后
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1996-2002 就读于得克萨斯大学健康医学中心及M.D. 安德森癌症研究中心，获得博士学位7 q) y4 p4 o9 C
5 k% |8 p3 E7 c1992-1996 就读于中国科学院微生物所，获得理学硕士学位' [! }. R$ h7 c4 J- v$ |/ Z" e) @
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1988-1992 就读于南开大学生物系，获得理学学士学位! w) U @" k$ _% s4 E% N% J, O) L
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研究方向：
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血液干细胞的分子和遗传调控研究) K* h9 @ y; I+ Z
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癌症干细胞的分子和遗传调控研究
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' g: f1 O2 |( L I. [3 K7 l癌症干细胞的靶向治疗研究
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发表文章：/ y: ^9 `4 h$ s: q& C
/ j$ P$ I" C' T; P) r' K1. Guo W, Suzanne S, Chen JY, Valamehr B, Mosessian S, Shi H, Dang NH, Garcia C, Theodoro MF, Varella-Garcia M, and Wu H. Suppression of leukemia development caused by PTEN loss. PNAS 2011; 108: 1409-1414.+ w5 X" `2 r! Z/ g2 M# M; O1 u4 I
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2. Guo W, Lasky JL, Chang CJ, Mosessian S, Lewis X, Xiao Y, Yeh JE, Chen JY, Iruela-Arispe LM, Varella-Garcia M, and Wu H. Multi-genetic events collaboratively contribute to Pten null leukemia stem cell formation. Nature 2008; 453: 529-533. 9 f- a, e# C9 Q- Q
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3. Drakos E., Rassidakis GZ, Leventaki V, Guo W, Medeiros LJ and Nagarajan L. Differential expression of the human MIXL1 gene product in Non-Hodgkin's and Hodgkin's lymphomas. Hum Pathol 2007; 38(3):500-7.6 |, u% g# B6 C, p
6 v: R! x( X; [2 B8 m/ e& b- Q) ]4. Guo W, Liang H and Nagarajan L. Amino terminal tyrosine phosphorylation of human MIXL1. J. Mol. Signaling 2006; 1:6.0 {" f" l+ H6 n$ [
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5. Yilmaz OH, Valdez R, Threisen BK, Guo W, Ferguson DO, Wu H, Morrison SJ. Pten dependence distinguishes hematopoietic stem cells from leukemia initiating cells. Nature 2006; 441(7092):475-82.
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6. Guo W, Lasky JL, and Wu H. Cancer stem cells. Pediatr Res 2006; 59: 59-64.6 g; B0 f) N; z# u: T5 E
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7. Stiles BL, Kuralwalla-Martinez C, Guo W, Gregorian C, Wang Y, Tian J, Magnuson MA, Wu H. Selective deletion of Pten in pancreatic β cells leads to increased islet mass and resistance to STZ-Induced diabetes. Mol Cell Biol 2006 Apr; 26(7):2772-81.
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* f8 ?; H- z7 |+ p# w8. Guo W, Chan AP, Liang H, Wieder ED, Molldrem JJ, Etkin LD and Nagarajan L. A human Mix-like homeobox gene MIXL shows functional similarity to Xenopus Mix.1. Blood 2002; 100:89-95.. Z0 x n, }& K3 Q1 E9 P6 Q
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9. Jiang Y, Liang H, Guo W, Kottickal LV and Nagarajan L. Differential expression of a novel C-terminally truncated splice form of SMAD5 in hematopoietic stem cells and leukemia. Blood 2000; 95(12): 3945-50.! x0 \! q! i+ F" h
4 |& L5 ~6 S/ Q3 @5 {, [1 D3 N. G, v10. Liang H, Guo W and Nagarajan L. Chromosomal mapping and genomic organization of an evolutionarily conserved zinc finger gene ZNF277. Genomics 2000; 66(2): 226-8.: @5 H+ z, l# q
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联系方式：
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- I- B3 n" k# E1 L' E电话：86-10-62782975
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6 N0 t4 U0 ~1 Q) `& s) \电邮：weiguo@mail.tsinghua.edu.cn, p7 e' W5 I! F" n# w0 ~8 r
; Z9 H' m, @- u! V+ m5. 那洁 副研究员、博士生导师
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9 [+ ^' m& b8 G C) zhttp://www.tsinghua.edu.cn/publi ... 5647477648623_.html. l! M) L9 w$ y' z/ Q3 h
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干细胞信号传导实验室
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1 W0 Z8 p$ d6 e6 ]英国发育生物学学会会员，国际干细胞研究学会会员。
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8 t5 k4 {7 t' x. t《Stem Cells》，《Stem Cell and Development》审稿人。
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6 Q- k; m5 e& B& P2010－至今，清华大学副教授。
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) i! P/ M- v# s; L2006－2010 Medical Research Council Stem Cell Career Development Fellow, University of Sheffield, 英国。
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! l* i C1 f/ o" V e2005－2006 Royal Society Research Fellow, University of Sheffield, 英国。3 b7 x) I8 ~" p" [
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2002－2005 博士后，Gurdon Institute, University of Cambridge, 英国。
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4 m) g, _ p# b0 I% e. q9 q1997－2002 University of Virginia，USA, 细胞生物学博士。6 K+ `1 _0 P- l4 {4 L; M
4 C- o% q+ b. {6 b: K$ d$ M* U1992－1997 北京医科大学基础医学系，医学学士。
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研究方向与手段：
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7 y2 {; \# u7 ^& b' f' D4 t# @' }1.调控人类胚胎干细胞自我更新和分化的信号传导机制。
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2.导致早期胚胎细胞和干细胞异常分裂，核型突变的原因与机制。( o0 f2 b+ j2 I
! I3 H+ U( Q! k8 c2 R3.运用RNA技术和基因工程，建立诱导多能干细胞（induced pluripotent stem cells)，引导干细胞定向分化。! Y- j' y+ ]& g# Z5 }$ ~+ l
3 z+ f% [9 q7 q' M. ?发表论文：( j2 X( L+ [7 U
' Z8 m5 l8 |" c1.Jordan R. Plews, Jian Liang Li, Mark Jones, Harry Moore, Chris Mason, Peter W. Andrews, JIE NA (corresponding author). Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. PLoS One 2010 Dec 30;5(12):e14397.
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2.JIE NA*, Jordan Plews, Jianliang Li, Patompon Wongtrakoongate, Timo Tuuri, Anis Feki, Peter W Andrews and Christian Unger*. Molecular Mechanism of Pluripotency and Reprogramming. Invited review for Stem Cell Research and Therapy 2010, 1:33 *co-corresponding authors.6 u" W7 w: e7 ^: P8 z0 B+ F
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3.Bedra Sharif*, JIE NA*, Karin Lykke-Hartmann, Stephen H. McLaughlin, Ernest Laue, David M Glover and Magdalena Zernicka-Goetz. The Chromosome Passenger Complex is required for Fidelity of Chromosome Transmission and Cytokinesis in Meiosis of mouse Oocytes. Journal of Cell Science 2010 Dec 15;123(Pt 24):4292-300. *co-first authors.
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4.JIE NA*, Miho K. Furue and Peter W. Andrews. Inhibition of ERK1/2 Prevents Neural and Mesendodermal Differentiation and Promotes Human Embryonic Stem Cell Self-renewal. Stem Cell Research. 2010 Sep;5 (2):157-69. *First and corresponding author.
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! h# n5 W3 T+ M8 @$ L! E( {/ Y5.Miho K. Furue, JIE NA, Jamie P Jackson, Tetsuji Okamoto, Mark Jones, Duncan Baker, Ryu-Ichiro Hata, Harry D.Moore, J. Denry Sato, Peter W. Andrews. Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium. PNAS 2008 Sep 9;105(36):13409-14.5 g( g# h; R" l6 i! v v# O& p! W3 c
+ U8 K6 m; _/ ^% l- ~7 Y4 ?- T6.JIE NA, Karin Lykke-Andersen, Maria Elena Torres-Padilla and Magdalena Zernicka-Goetz. Dishevelled Proteins Regulate Cell Adhesion in Mouse Blastocyst and Serve to Monitor Changes in Wnt Signaling. Developmental Biology 2007 Feb 1;302(1):40-9
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7.JIE NA and Magdalena Zernicka-Goetz. Asymmetric Positioning and Organisation of the Meiotic Spindle of Mouse Oocytes Requires CDC42 Function. Current Biology 2006 Jun 20;16(12):1249-54
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; F0 ~7 L6 {+ S* E" {) f8.Dionne Gray, Berenika Plusa, Karolina Piotrowska, JIE NA, Brian Tom, David M. Glover and Magdalena Zernicka-Goetz. First Cleavage of the Mouse Embryo Responds to Change in Egg Shape at Fertilization. Current Biology 14, 397-405, 2004.4 ?5 N4 a3 ]! J' a/ d
6 ^# N: }0 i( W3 f, J/ N; C9.JIE NA, Mungo Marsden and Douglas W. DeSimone. Differential Regulation of Cell Adhesive Functions by Integrin ？ Cytoplasmic Tails In vivo. Journal of Cell Science 116, 2333-2343, 2003.. k5 r2 |; x' U9 x
4 g! `" r9 z( N# a10.JIE NA, Douglas DeSimone. Differential Regulation of Cell Adhesive Behaviour by Integrin ？ Cytoplasmic Tails. Molecular Biology of the Cell, Vol. 12 Suppl:463a, November 2001./ b# X% O3 V, h1 ^& U
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联系方式：7 n7 `2 R' Y, m
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电子邮件：jie.na@tsinghua.edu.cn
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发表于 2011-8-3 09:23
