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I really appreciate your commentary. ; Z, \/ b. J: S5 y$ q7 X
Actually, since Dr. Martin Evans first isolated murine ESCs from 129Sv strain, there is a long-term problem for murine ESCs culturing. The batch variation problem for serum is addressed a lot by researchers around the world. But for other cell culturing, even some very tenellous primary cell types, such a problem is not as prominent as in mESCs. What's the most optimal culture condition for murine ESCs (for human ESCs, the problem is much more complicated)? Dr. Austin Smith and his postdoc Qi-long Ying (now hold a position at Univ South Carolina) contributed a lot to define the critical and essential components to culture murine ESCs. Although their 2i or 3i medium is sold by some companies (such as invitrogen) now, it can only be used to culture some specific strains of ESCs and the adhesion ability of these cells is also not good. But if we start from an instable point, these variations are reasonable.
# t/ H- r5 p$ W7 y3 ~2 F% d; GIt's just my two cents. |
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