iPS、胚胎干与直接转分化的“三国演义”

zhenhua

回复 tpwang 的帖子7 m3 n# |/ v, @1 v8 f

" b8 R, A$ P5 ^$ E2 ?; w/ L人ES无法用四倍体互补实验。
1 ^4 S. [+ }+ N, A8 Y; C/ N最近Rudolf Jaenischa发了一篇Human embryonic stem cells with biological and: d5 l( K$ I7 e$ |- j* J
epigenetic characteristics similar to those of mouse ESCs的文章。6 w* X9 d$ _/ ?8 {; ^
通过ectopic induction of Oct4, Klf4, and Klf2 factors combined with LIF and inhibitors of glycogen synthase kinase 3β& O, r* W: L" {. {1 i. Z. X& x) X
(GSK3β) and mitogen-activated protein kinase (ERK1/2) pathway，是hES与mES达到比较类似的程度。
8 s3 ~9 u4 z) W- P* x6 e那可不可以利用这种方法，来验证hES和hiPS多能性的标准？