关于iPS和畸胎瘤？

小刀

我做的“iPS”，SSEA-3/4,TRA-1-80/61检测都为阳性，可裸鼠形不成畸胎瘤，不知道为什么？请前辈指点！

待鹤

Teratoma Formation( c/ f5 ~# d' g  U/ p
The cells were harvested by collagenase IV treatment, collected into3 R2 H( h; C8 m/ {
tubes, and centrifuged, and the pellets were suspended in DMEM/
/ m( O& ^7 ^% N; U, QF12. One quarter of the cells from a confluent 100 mm dish was injected
0 z* i1 I  ^5 u6 {subcutaneously to dorsal flank of a SCID mouse (CREA, Japan).
, m0 \* ?! \" v# G; v! {0 SNine weeks after injection, tumors were dissected, weighted, and fixed: u* Q" Z# N' u! x) W
with PBS containing4%paraformaldehyde. Paraffin-embedded tissue& `8 z  ?' h" |* D- K' k
was sliced and stained with hematoxylin and eosin.
' j* e1 b/ o9 ~  \, j3 P! d$ _6 y上面是Yamanaka的操作方法，是不是细胞太少了？

小刀

可能是吧，改天再试试

hansey

裸鼠有时候也挺难成瘤的，可以用SCID鼠试试