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APPENDIX A0 O: o0 v+ G: O- H W' K
OVERVIEW OF FIBROBLAST MEDIA* j B4 D8 G! ?# c
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500 ml Bottles (except where indicated)
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CC-3131 FBM? Fibroblast Basal Medium (no growth factors)
! e: n# h" r- P' L( L+ D3 T4 F/ T6 WCC-3130 FGM? BulletKit? Kit which contains a 500 ml bottle of FBM?, (CC-3131) and FGM? SingleQuots? (CC-4134) which contains all of the supplements listed below, conveniently packaged as single-use aliquots (amounts indicate concentration of each SingleQuot?)
; j0 C5 r! u: w1 X1 mg/ml hFGF (human recombinant Fibroblast Growth Factor) (CC-4065) 0.5 ml4 P: i: t, G) q0 ?& Y8 X. O5 N
5 mg/ml Insulin (CC-4021) 0.5 ml
, I( Z- Y7 E# n50 mg/ml Gentamicin, 50 mg/ml Amphotericin-B (CC-4081) 0.5 ml
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CC-3132 FGM?-2 BulletKit? Kit which contains a 500 ml bottle of FBM?, (CC-3131) and FGM?-2 SingleQuots? (CC-4126) which contains all of the supplements listed below, conveniently packaged as single-use aliquots (amounts indicate concentration of each SingleQuot?)
9 {- |" X6 b0 ^; X7 }% n1 mg/ml hFGF (human recombinant Fibroblast Growth Factor) (CC-4065) 0.5 ml
( b: a' b2 U) e. f7 `* ?5 mg/ml Insulin (CC-4021) 0.5 ml ) \' `3 B# }1 Q- e
50 mg/ml Gentamicin, 50 mg/ml Amphotericin-B (CC-4081) 0.5 ml ! ]) e8 {2 O) m4 S; m W$ z% e, |, S
10 ml FBS (Fetal Bovine Serum) (CC-4101)
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CC-3134 FGLM™ Custom FGM? Labeling Medium BulletKit? (500 ml) that consists of the following: 5 ?: s: O4 m8 W9 P; k' b% [
CC-3133 FBLM™ Fibroblast Basal Labeling Medium, without the following nutrients: Myo- Inositol, Thymidine, Proline, Isoleucine, Leucine, Methionine, and Cysteine.
$ |8 ~4 J8 ~& d& t CC-4153 FGLM™ SingleQuot? Kit, FGM? labeling SingleQuots? consisting of the following:
/ ?/ I- C& K6 | M, p7 u( j6 a3.513 mg/ml L-Cysteine(CC-4069)2 ml* j) P V" X5 u' c1 C8 V6 X9 A
6.559 mg/ml L-Isoleucine(CC-4045) 1 ml9 Z$ c1 M/ t4 ]
9.01 mg/ml Myo-Inositol(CC-4050) 1 ml
3 k" ~/ ?3 Y* _- O9.838 mg/ml L-Leucine(CC-4046)2 ml
0 x7 S* e, a" P2 I2.238 mg/ml L-Methionine(CC-4047) 1 ml
: s6 J& q8 @, y$ L9 ^4 h, O' ]$ R2.878 mg/ml L-Proline(CC-4048)1 ml& ?- ^) e$ [$ n; j; }' I( |
0.036 mg/ml L-Thymidine(CC-4049) 1 ml
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9 Y/ p7 p' L ` CC-4134 FGM? SingleQuots? (See CC-3130)
+ Y9 ]: Q) c/ ^5 R5 g) i0 N' s" nCC-3135 FGLM™-2 Custom FGM?-2 Labeling Medium BulletKit? (500 ml) that consists of the following: _ I6 L* ?2 I/ d. K0 b V- V
CC-3133 FBLM™ Fibroblast Basal Labeling Medium, without the following nutrients: Myo- Inositol, Thymidine, Proline, Isoleucine, Leucine, Methionine, and Cysteine. % B. r; o i9 w
CC-4153 FGLM™ SingleQuot? Kit, FGM? labeling SingleQuots? consisting of the following:
# R2 m( @, d# {; v; a7 C" d+ p3.513 mg/ml L-Cysteine(CC-4069)2 ml
_$ ~+ w& _( @, _$ ~; j6.559 mg/ml L-Isoleucine(CC-4045) 1 ml
( s* E: l' p5 w3 `/ ^& h9.01 mg/ml Myo-Inositol(CC-4050) 1 ml* ?- |! h( Y5 q- g7 G
9.838 mg/ml L-Leucine(CC-4046)2 ml5 s, f8 r2 t/ V4 ]9 ~" s! U
2.238 mg/ml L-Methionine(CC-4047) 1 ml6 Q- h Q/ z* N% U% \- K
2.878 mg/ml L-Proline(CC-4048)1 ml& D( P) ~4 k* F) W$ G N$ K3 F
0.036 mg/ml L-Thymidine(CC-4049) 1 ml
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" }- q4 p$ ]& B) J/ k- x. ^8 o CC-4126 FGM?-2 SingleQuots? (See CC-3132) 0 C' T( p) `) L9 ~* Q
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NOTE: All Clonetics? Media can be custom formulated to meet your research needs. Contact your Technical Specialist for more information. |
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