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- 积分
- 266
- 威望
- 266
- 包包
- 863
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M2 medium: Dissolve 0.356 g KCl (cat. no. P5405; Sigma), 0.252 g CaCl2·2H2O
4 n! \' c5 G' u9 t G( y* ~$ X; w$ X(cat. no. C7902; Sigma), 0.162 g KH2PO4 (cat. no. P5655; Sigma), 0.293 g
; C9 ^! O! }8 l1 ]MgSO4·7H2O (cat. no. M1880; Sigma), 0.349 g NaHCO3 (cat. no. S5761; Sigma),
. x3 i2 i; ]& n! z( J* H1 L4.969 g HEPES (cat. no. H9136; Sigma), 2.610 g sodium lactate (cat. no. L7900; A& n0 D" R _4 R/ Z
Sigma), 0.036 g sodium pyruvate (cat. no. P5280; Sigma), 1.0 g of glucose (cat. no.
5 W7 W" U0 {' B. U( K- R. _G6138; Sigma), 4.0 g bovine serum albumin (cat. no. A6003; Sigma), 0.060 g
; p/ w2 p m, i% N6 U9 |/ c7 Q9 qpenicillin G potassium salt (cat. no. P4687; Sigma), 0.050 g streptomycin sulfate6 F3 P9 ^3 e9 y7 C( K% v: _) `
(cat. no. S1277; Sigma), 0.010 g phenol red (cat. no. P0290; Sigma) in 800 mL of
- E8 Z6 c e; GMilli-Q water. Adjust to pH 7.4 and 280 mosM, and make up the volume to 1 L
{- b% c/ j: x5 Wwith Milli-Q water. |
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总评分: 威望 + 10
包包 + 10
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发表于 2009-9-21 09:25
发表于 2009-9-26 07:48
